Dithiothreitol

Référence 20710.03

Conditionnement : 5G

Marque : SERVA Electrophoresis

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(DTT, Cleland's reagent, Dimercapto-2,3-butanediol)
C4H10O2S2Mr 154.25CAS [3483-12-3]

Dithiothreitol, as well called DTT or Cleland's reagent, quantitatively reduces disulfide groups (1) and is effective in sample buffers for reducing protein disulfide bonds prior to SDS gel electrophoresis (SDS-PAGE). The reducing thiol agent cleaves disulfide bonds between cysteine residues, thus totally unfolding proteins to allow a separation according to the molecular weight. It may also be used in lysis buffer in protein sample preparations. It is not only less pungent and less toxic than 2-mercaptoethanol, but as well a seven fold lower concentration of DTT (100 mM) than of 2-mercaptoethanol (5 % v/v, 700 mM) is needed. Oxidized form of DTT max. 0.5 %. Hygroscopic.

Assay (titr.)
MP
min. 99.0 %
40 - 45 °C


References:
  1. Cleland, W.W. (1964) Biochemistry 3, 480-2
WARNING
Hazard Statements H302 - H315 - H319 - H335
Precautions P264 - P280
Reaction P301 +P312 - P302 +P352 - P304 +P340 - P305 +P351 +P338

EINECS: 222-468-7WGK: 3LHS: 29309098
Storage Temperature: +2 °C to +8 °C

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