Dolichos biflorus (DBA) (Biotin)

Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms.

Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.

Pure Dolichos biflorus lectin (DBA) from horse gram, Biotin conjugated.

Methyl-2-acetamido-2-deoxy-D-galactose.

Terminal alpha-D-GalNAc.

4ug/ml will agglutinate human type A1 cells. As much as 200ug/ml is needed to agglutinate type A2 cells.

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.  For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Purified

~1mg/ml

Supplied as a liquid in PBS, pH 7.3. Labeled with Biotin.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.