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EGFR Protein, Mouse, Recombinant (His)

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Référence NB-64-57772-50ug

Conditionnement : 50ug

Marque : Neo Biotech

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EGFR Protein, Mouse, Recombinant (His)

(Synonyms: Wa5, wa-2, wa2, Errp, Errb1, Erbb, epidermal growth factor receptor, AI552599, 9030024J15Rik) Copy Product Info

Synonyms: Wa5, wa-2, wa2, Errp, Errb1, Erbb, epidermal growth factor receptor, AI552599, 9030024J15Rik

Catalog No. TMPY-02676 Copy Product Info

EGFR Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 70.8 kDa and the accession number is Q01279.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:SDS-PAGE: 95.8%; SEC-HPLC: 99.8%

Appearance:Lyophilized powder

Color:White

Product Information

Bioactivity

Bioactivity	1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind human EGF-Fc , The EC50 of human EGF-Fc is 60-90 ng/mL. 3. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind mouse EGF-Fc , The EC50 of mouse EGF-Fc is 70-100 ng/mL.
Description	EGFR Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 70.8 kDa and the accession number is Q01279.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q01279
Construction	A DNA sequence encoding the extracellular domain of mouse EGFR (Q01279) (Met 1-Ser 647) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Leu 25
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC. ,
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	Wa5, wa-2, wa2, Errp, Errb1, Erbb, epidermal growth factor receptor, AI552599, 9030024J15Rik
Research Background	As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight	70.8 kDa (predicted); 100 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

AI-552599AI 552599

Related Tags: EGFR Protein, Mouse, Recombinant (His) chemical structure | EGFR Protein, Mouse, Recombinant (His) molecular weight