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> F(ab')2 Human lambda antibody (Peroxidase)

F(ab')2 Human lambda antibody (Peroxidase)

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Conditionnement : 1mg

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    • F(ab')2 Human lambda antibody (Peroxidase)

    F(ab')2 Human lambda antibody (Peroxidase)

    Catalog Number: orb348187

    Catalog Numberorb348187
    CategoryAntibodies
    DescriptionF(ab')2 Human lambda antibody (Peroxidase)
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsELISA, IHC, WB
    ReactivityHuman
    IsotypeIgG F(ab')2
    ImmunogenHuman lambda light chain
    Concentration1.0 mg/mL
    Dilution rangeELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
    Form/AppearanceLyophilized
    PurityF(ab')2 Human lambda chain Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
    ConjugationHRP
    StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    Alternative namesGoat F(ab')2 Anti-Human λ (lambda chain) Antibody
    NoteFor research use only
    Application notesF(ab')2 Anti-Human lambda chain Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.
    Expiration Date12 months from date of receipt.
    F(ab')2 Human lambda antibody (Peroxidase)

    Western Blot of Goat anti-Human λ (Lambda chain) Peroxidase Conjugated Secondary Antibody. Lane 1: Human λ. Lane 2: Human κ. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Human λ. Other band(s): Human λ splice variants and isoforms.