Fab Anti-MOUSE IgG (H&L) (GOAT) Antibody

Référence 810-1102

Conditionnement : 1mg

Marque : Rockland Immunochemicals

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Specifications for Fab Mouse IgG (H&L) Antibody

Product Details

Goat Fab Anti-Mouse IgG (H&L) Antibody - 810-1102
Goat Fab Anti-Mouse IgG Antibody
Goat
IgG (H&L)
Polyclonal
IgG Fab

Target Details

Mouse
Native Protein
Anti-Mouse IgG (H&L) antibody was produced by repeated immunization with mouse IgG whole molecule in goat.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).

Application Details

Dot Blot, ELISA
IHC  - View References
Fab Anti-Mouse IgG has been tested by ELISA and dot blot and is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Formulation

1.0 by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Wet Ice
Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

Background

Fab Anti-Mouse IgG (H&L) Antibody generated in goat detects Mouse IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

References (6)

(). Biogenesis of large dense core vesicles in mouse chromaffin cells. Traffic.
Applications
IHC, ICC, Histology
PubMed
(). Background-free Detection of Mouse Antibodies on Mouse Tissue by Anti-isotype Secondary Antibodies. J Histochem Cytochem.
Applications
IHC, ICC, Histology
PubMed
(). Paralogs of the Calcium-Dependent Activator Protein for Secretion Differentially Regulate Synaptic Transmission and Peptide Secretion in Sensory Neurons. Frontiers in Cellular Neuroscience
Applications
IHC, ICC, Histology
PubMed
(). Trpm5 expression in the olfactory epithelium. Mol Cell Neurosci.
Applications
IHC, ICC, Histology
PubMed
(). Reelin protects against amyloid β toxicity in vivo. Science Signaling
Applications
IHC, ICC, Histology
PubMed
(). Synergistic Bcl-2 inhibition by ABT-737 and cyclosporine A. Apoptosis.
Applications
IHC, ICC, Histology
PubMed

Related Protocols

Adherent Cell Lysis Protocol
ELISA Protocol
General Immunoblotting Protocol
Heat-induced Antigen Retrieval Protocol
Immunohistochemistry (IHC) Protocol
Protease-induced Antigen Retrieval Protocol
Sandwich ELISA Protocol for Collagen
Staining Paraffin Sections by PAP Procedure
Suspension Cultured Cell Lysis Protocol

Certificate of Analysis Lookup

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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