FGF15 ELISA Kit (Mouse)
Référence OKEH02994
Conditionnement : 96Wells
Marque : Aviva Systems Biology
Predicted Species Reactivity | Mouse, Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology FGF15 ELISA Kit (Mouse) (OKEH02994) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fgf15 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Fgf15 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fgf15 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 78-5000pg/ml | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.9% (n = 20) Mean Inter-assay CV%: < 7.7% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
Sensitivity | 39.4 pg/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Mouse Fibroblast growth factor 15 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | Fgf15 |
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Gene Full Name | fibroblast growth factor 15 |
Alias Symbols | FGF-15;FGF19;Fgf8a;fibroblast growth factor 15;fibroblast growth factor 8a. |
NCBI Gene Id | 14170 |
Protein Name | O35622 |
Description of Target | Fibroblast growth factor 15 |
Uniprot ID | https://www.uniprot.org/uniprot/O35622 |
Protein Accession # | NP_032029.1 |
Nucleotide Accession # | NM_008003.2 |
Protein Size (# AA) | 218 |
Molecular Weight | 25 kDa |