Orb511130-6ml | Fixative Clinisciences

Fixative

SKU: orb511130

Description

ICT’s Fixative is a formaldehyde solution designed to cross-link intracellular components. It will not interfere with the fluorescence labels used on our fluorescent detection probes like FLICA, Magic Red, and FLISP.

Images & Validation

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Application Notes	1. Add PI to the cell sample media at 0.5% v/v. For example, add 1.5 µL PI to 300 µL of cells. 2. Incubate 5-10 minutes at room temperature. 3. Visualize with a fluorescence microscope with excitation at 488 nm and emission at 635 nm. 4. Alternatively, cells can be analyzed with a flow cytometer using a blue laser at 488 nm or a green laser at 525 nm. Read fluorescent output in FL-2 or FL-3. 5. When bound to DNA, the maximum absorption is 535 nm and the maximum emission is 617.

Application Notes

1. Add PI to the cell sample media at 0.5% v/v. For example, add 1.5 µL PI to 300 µL of cells. 2. Incubate 5-10 minutes at room temperature. 3. Visualize with a fluorescence microscope with excitation at 488 nm and emission at 635 nm. 4. Alternatively, cells can be analyzed with a flow cytometer using a blue laser at 488 nm or a green laser at 525 nm. Read fluorescent output in FL-2 or FL-3. 5. When bound to DNA, the maximum absorption is 535 nm and the maximum emission is 617.

Key Properties

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Storage & Handling

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Storage	2-8°C
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Alpha-(trichloromethyl)benzyl acetate [orb2939510]
CAS Number:
90-17-5
MW:
267.54
Formula:
C₁₀H₉Cl₃O₂
Sizes Available: 10 g, 25 g, 50 g
Bouin's solution [orb2940714]
Sizes Available: 10 L, 5 L, 1 l
Chromium(Ⅲ) potassium sulfate dodecahydrate [orb2941088]
CAS Number:
7788-99-0
MW:
499.403
Formula:
CrH₂₄KO₂₀S₂
Sizes Available: 100 g, 25 g
Tissue Fixative Fluid [orb1566670]
Sizes Available: 25 x 15 ml

Fixative

Référence orb511130-6ml

Marque : Biorbyt

Fixative

Description

Images & Validation

Key Properties

Storage & Handling

Alpha-(trichloromethyl)benzyl acetate [orb2939510]

Bouin's solution [orb2940714]

Chromium(Ⅲ) potassium sulfate dodecahydrate [orb2941088]

Tissue Fixative Fluid [orb1566670]

Protocol Information

CAS Number:	90-17-5
MW:	267.54
Formula:	C₁₀H₉Cl₃O₂

CAS Number:	7788-99-0
MW:	499.403
Formula:	CrH₂₄KO₂₀S₂