FKBP52 antibody
Référence orb147210-100ug
Conditionnement : 100ug
Marque : Biorbyt
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FKBP52 antibody
Catalog Number: orb147210
| Catalog Number | orb147210 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal to FKBP52 (APC). Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones. One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR co-chaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins. FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex. The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90. Although FKBP52 and FKBP51 share 75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90-binding characteristics (3, 5). Also, whereas FKBP51 typically has a role with the progesterone receptor, FKBP52 has been found to be linked to the progesterone, androgen and glucocorticoid receptors.. |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | Hi52C |
| Tested applications | ICC, IHC, IP, WB |
| Reactivity | Canine, Hamster, Human, Mouse, Rat |
| Isotype | IgG2a |
| Immunogen | Synthetic peptide corresponding to the residues of human FKBP52 |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:2000), IHC (1:250), ICC/IF (1:1000), IP (5µg) |
| Conjugation | APC |
| MW | 52kDa |
| Target | FKBP52 |
| Entrez | 2288 |
| UniProt ID | Q02790 |
| NCBI | NP_002005.1 |
| Storage | Conjugated antibodies should be stored at 4°C |
| Buffer/Preservatives | PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated |
| Alternative names | FK506 binding protein 4 antibody, FKBP4 antibody, |
| Note | For research use only |
| Application notes | 0.5 µg/ml was sufficient for detection of FKBP52 in 20 µg total protein using WB by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary. |
| Expiration Date | 12 months from date of receipt. |