Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> GABA-A Receptor Alpha 2 Antibody

GABA-A Receptor Alpha 2 Antibody

Imprimer Imprimer
Identifiez-vous

Référence ASM10318-STR-100

Conditionnement : Onrequest

Marque : Abcepta

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

 
  • ICC/IF - GABA-A Receptor Alpha 2 Antibody ASM10318
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha2 Monoclonal Antibody, Clone S399-19 (ASM10318). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha2 Monoclonal Antibody (ASM10318) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABA-A Receptor Alpha2 Antibody (D) Composite.
  • WB - GABA-A Receptor Alpha 2 Antibody ASM10318
    Western Blot analysis of Mouse Brain showing detection of ~55 kDa GABA A Receptor Alpha 2 protein using Mouse Anti-GABA A Receptor Alpha 2 Monoclonal Antibody, Clone S399-19 (ASM10318). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 µg. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 2 Monoclonal Antibody (ASM10318) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa. Other Band(s): ~ 37 kDa, ~50 kDa, ~70 kDa.
  • WB - GABA-A Receptor Alpha 2 Antibody ASM10318
    Western Blot analysis of Rat Brain showing detection of ~55 kDa GABA A Receptor Alpha 2 protein using Mouse Anti-GABA A Receptor Alpha 2 Monoclonal Antibody, Clone S399-19 (ASM10318). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 10 µg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 2 Monoclonal Antibody (ASM10318) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~55 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF
Primary Accession P23576
Other Accession NP_001129251.1
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat GABA-A Receptor Alpha2 Monoclonal IgG1
Target/Specificity Detects ~55kDa. Does not cross-react with GABA-A Receptor Alpha1.
Other Names GABA A receptor subunit alpha 2 Antibody, GABRA2 Antibody, Gamma aminobutyric acid A receptor Alpha 2 Antibody, GBRA2_Human Antibody
Clone Names S399-19
Immunogen Fusion protein amino acids 350-385 (Cytoplasmic C-terminus) of rat GABA-A Receptor Alpha2
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:100 dilution of SMC-486 was sufficient for detection of GABA-A R, Alpha2 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm