GENIUS™ Nuclease [9025-65-4]
Référence sc-202391
Conditionnement : 25KU
Marque : Santa Cruz Biotechnology
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GENIUS™ Nuclease (CAS 9025-65-4)
Application:
Pureté:
≥90%
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
* Consulter le Certificat d'Analyses pour les données spécifiques à un lot (incluant la teneur en eau).
ACCÈS RAPIDE AUX LIENS
Informations pour la commande
Références bibliographiques
Description
Information Technique
Données de Sécurité
SDS & Certificat d’Analyses
GENIUS™ Nuclease is a solubilized version of GENIUS™ Nuclease DMF filed. The GENIUS™ Nuclease enzyme is a genetically engineered endonuclease from Serratia marcescens. GENIUS™ Nuclease, also called Golden nuclease, is a highly useful endonuclease for removal of nucleic acids from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts.
GENIUS™ Nuclease is a genetically engineered endonuclease from Serratia marcescens. GENIUS™ Nuclease, also called Golden nuclease, is a highly useful endonuclease for removal of nucleic acids from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts.
The enzyme completely digests nucleic acids to 5′-monophosphate terminated oligonucleotides 2–5 bases in length. Although the nuclease is capable of cleavage at nearly all positions along a nucleic acid chain, sequence-dependent preferences have been demonstrated. The enzyme prefers GC-rich regions in dsDNA while avoiding d(A)/d(T)-tracts. GENIUS™ Nuclease is ideal for a wide variety of applications where complete digestion of nucleic acids is desirable. Digests native or heat-denatured DNA and RNA. This can also be used for the removal of nucleic acid from protein samples.
Unit definition:
One unit of GENIUS™ Nuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min, which corresponds to complete digestion of 37 μg of DNA. Note that 1 KU = 1000 units.
GENIUS™ Nuclease is a genetically engineered endonuclease from Serratia marcescens. GENIUS™ Nuclease, also called Golden nuclease, is a highly useful endonuclease for removal of nucleic acids from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts.
The enzyme completely digests nucleic acids to 5′-monophosphate terminated oligonucleotides 2–5 bases in length. Although the nuclease is capable of cleavage at nearly all positions along a nucleic acid chain, sequence-dependent preferences have been demonstrated. The enzyme prefers GC-rich regions in dsDNA while avoiding d(A)/d(T)-tracts. GENIUS™ Nuclease is ideal for a wide variety of applications where complete digestion of nucleic acids is desirable. Digests native or heat-denatured DNA and RNA. This can also be used for the removal of nucleic acid from protein samples.
Unit definition:
One unit of GENIUS™ Nuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min, which corresponds to complete digestion of 37 μg of DNA. Note that 1 KU = 1000 units.
References:
- Disulfide bonds are required for Serratia marcescens nuclease activity. | Ball, TK., et al. 1992. Nucleic Acids Res. 20: 4971-4. PMID: 1329033
- The extracellular nuclease gene of Serratia marcescens and its secretion from Escherichia coli. | Ball, TK., et al. 1987. Gene. 57: 183-92. PMID: 3319779
- 2.1 A structure of Serratia endonuclease suggests a mechanism for binding to double-stranded DNA. | Miller, MD., et al. 1994. Nat Struct Biol. 1: 461-8. PMID: 7664065
- A new mass spectrometric approach to detect modifications in DNA. | Janning, P., et al. 1994. Rapid Commun Mass Spectrom. 8: 1035-40. PMID: 7696699
- Identification of catalytically relevant amino acids of the extracellular Serratia marcescens endonuclease by alignment-guided mutagenesis. | Friedhoff, P., et al. 1994. Nucleic Acids Res. 22: 3280-7. PMID: 8078761
- Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. | Molloy, MP., et al. 1998. Electrophoresis. 19: 837-44. PMID: 9629924
- Improved protein solubility in two-dimensional electrophoresis using tributyl phosphine as reducing agent. | Herbert, BR., et al. 1998. Electrophoresis. 19: 845-51. PMID: 9629925
Utilisation :
The activity of any unknown nuclease can be determined from a single measurement by means of the standard curve. The specific activity of GENIUS™ Nuclease is 1.3×106 ~ 1.5×106 unit/mg protein.
Formulation :
≥250 units/µL supplied in solution containing 20 mM Tris HCl (pH 8.0), 2 mM MgCl2, 20 mM NaCl and 50% glycerol.
État Physique :
Liquid
Dérivé de :
Recombinant protein expressed in Escherichia coli
STOCKAGE :
Store at -20° C
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
WGK Allemagne :
1
Numéro MDL :
MFCD00131010
Numéro CE :
231-791-2
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Region
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SAMPLE Certificate of Analysis (COA)
GENIUS™ Nuclease | CAS 9025-65-4 SAMPLE Certificate of AnalysisCERTIFICAT D'ANALYSE
Numéro de catalogue
Numéro de Lot
Envoyer
SAMPLE Certificate of Analysis (COA)
Catalog Number:sc-202391
Lot Number:SAMPLE
Product Name:GENIUS™ Nuclease
Native Source:Recombinant protein expressed in Escherichia coli
| Test | Specification | Results |
|---|---|---|
| Activity | Biological activity: > 250U/µL | 625 U/µL |
| Appearance | Clear, no visible particles | Complies |
| Purity (SDS-PAGE) | > 95% | Complies |
| Specific Activity | ≥ 1.0 x106 unit/mg | Complies |
| Endotoxin by LAL | <1 EU/µg | Complies |
| Protease | Not detectable | Complies |
| Sterility | < 0.1 CFU/ml | Complies |
TEST CONDITIONS
Exp. Date: 12/14/2027