GFAP antibody
Référence GTX108711-25ul
Conditionnement : 25ul
Marque : Genetex
Host | Rabbit |
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Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, IHC-Fr, IHC |
Reactivity | Human, Mouse, Rat |
Package | 100 μl, 25 μl |
APPLICATION
Application Note
Application | Recommended Dilution |
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WB | 1:5000-1:50000 |
ICC/IF | 1:100-1:1000 |
IHC-P | 1:100-1:1000 |
IHC-Fr | 1:100-1:1000 |
IHC | Assay dependent |
Calculated MW
Positive Control
Predict Reactivity
PROPERTIES
Form
Buffer
Preservative
Storage
Concentration
Antigen Species
Immunogen
Purification
Conjugation
RRID
Note
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Cellular Localization
Background
Database
DATA IMAGES
GTX108711 IHC-Fr Image GFAP antibody detects GFAP protein at retinal ganglion cell layer by immunohistochemical analysis. |
GTX108711 WB ImageNon-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX108711 IHC-P ImageGFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.GFAP stained by GFAP antibody (GTX108711) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX108711 IHC-P ImageGFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.GFAP stained by GFAP antibody (GTX108711) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX108711 IHC-Fr Image GFAP antibody detects GFAP protein expression by immunohistochemical analysis. |
GTX108711 IHC-P Image GFAP antibody detects GFAP protein at cytoplasm in rat brain by immunohistochemical analysis. |
GTX108711 ICC/IF ImageGFAP antibody detects GFAP protein at glia cells by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GFAP stained by GFAP antibody (GTX108711) diluted at 1:500.Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920). |
GTX108711 IHC-Fr ImageGFAP antibody detects GFAP protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse spinal cord.Green: GFAP stained by GFAP antibody (GTX108711) diluted at 1:300.Blue: Hoechst 33342 staining. |
GTX108711 IHC-P ImageGFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.GFAP stained by GFAP antibody (GTX108711) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX108711 IHC-Fr Image GFAP antibody detects GFAP protein expression by immunohistochemical analysis. |
GTX108711 IHC-P ImageGFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.GFAP stained by GFAP antibody (GTX108711) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
GTX108711 ICC/IF ImageGFAP antibody detects GFAP protein at glia cells by immunofluorescent analysis.Sample: DIV10 rat E18 primary hippocampal neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GFAP stained by GFAP antibody (GTX108711) diluted at 1:250.Red: Tau, a cytoskeleton marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920). |
GTX108711 WB ImageVarious tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX108711 WB ImageVarious whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX108711 WB ImageVarious whole cell and tissue extracts were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
Application Reference
REVIEW
GFAP antibody Cat. No. GTX108711
Rating | | ( Average 5 based on 10 users reviews) |
Western Blot(WB) | | ( Average 5 based on 4 users reviews) |
Immunocytochemistry/ Immunofluorescence(ICC/IF) | | ( Average 5 based on 3 users reviews) |
IHC (Frozen sections)(IHC-Fr) | | ( Average 5 based on 3 users reviews) |
SDS | |
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PBS.pdf | |
Glycerol.pdf | |
Proclin.pdf |