Specifications

Product Data
Applications	IF, IHC, WB
Recommended Dilution	Western Blot. Immunocytochemistry. Immunohistochemistry. Recommended Dilutions: 1/10000-1/20000 for Western blots. 1/2000-1/5000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Quality Control: This antibody was analyzed by Immunohistochemistry (at dilution of 1/5000) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent.
Reactivities	Human, Mouse, Rat
Host	Chicken
Isotype	IgY
Clonality	Polyclonal
Immunogen	Recombinant Human GFAP (expressed in bacteria). After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks.
Specificity	Recognizes Glial Fibrillary Acidic Protein (GFAP).
Formulation	10 mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified (filter sterilized) IgY fraction.
Concentration	2 mg/ml (based on absorbance at 280 nm)
Purification	Affinity Chromatography using a peptide column.
Conjugation	Unconjugated
Storage	Store undiluted at 2-8°C.
Stability	Shelf life: one year from despatch.
Gene Name	glial fibrillary acidic protein
Database Link	Entrez Gene 14580 MouseEntrez Gene 24387 RatEntrez Gene 2670 Human UniProt ID P14136
Background	Human GFAP is a 49,749 dalton protein (432 amino acids) expressed by astrocytes of the central nervous system. GFAP is an intermediate filament protein and acts as an intra-cellular structural component of the astrocytic cytoskeleton. During embryonic and fetal life, GFAP is also expressed by radial glial cells of the CNS. Rare mutations of the GFAP gene in humans result in Alexander’s disease, one of the leukodystrophies.
Synonyms	Glial Fibrillary Acidic Protein
Reference Data
Protein Families	ES Cell Differentiation/IPS