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> GIT1 Antibody

GIT1 Antibody

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Référence ASM10247-STR-100

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  • ICC/IF - GIT1 Antibody ASM10247
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (ASM10247). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (ASM10247) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GIT1 Antibody (D) Composite.
  • WB - GIT1 Antibody ASM10247
    Western Blot analysis of Rat brain membrane lysate showing detection of GIT1 protein using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (ASM10247). Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (ASM10247) at 1:1000.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF, IP
Primary Accession Q9Z272.1
Other Accession NP_1140021.1
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat GIT1 Monoclonal IgG1
Target/Specificity Detects ~90kDa. Does not cross-react with GIT2.
Other Names ARF GAP GIT1 Antibody, ARF GTPase-activating proteinAntibody, CAT1 Antibody, GRK-interacting protein 1 Antibody, ARF GTPase-activating protein GIT1 Antibody, Cool-associated and tyrosine-phosphorylated protein 1 Antibody, G protein-coupled receptor kinase-interactor 1 Antibody, GIT 1 Antibody
Clone Names S39B-8
Immunogen Fusion protein amino acids 375-770 (C-terminus) of rat GIT1
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-413 was sufficient for detection of GIT1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm