Glutathione Fluorometric Assay Kit

Glutathione is a low-molecular-weight intracellular thiol. It is essential for protecting mammalian cells from oxidative and nitrosative stress. In the early stages of apoptosis, abolished glutathione levels was observed. The Glutathione Fluorometric Assay Kit gives a simple in vitro assay for detecting total glutathione changes in apoptosis and other samples. This kit applies a dye called monochlorobimane (MCB) that forms an adduct with glutathione. When MCB bound to glutathione of reduced or oxidized form, the dye emits blue fluorescence (Ex/Em = 360 nm/461 nm). The reaction is catalyzed by glutathione S-transferase and the level of total glutathione can be detected by fluorometer or fluorometric plate reader.