Goat anti-CFTR
Référence EB06694
Conditionnement : 100ug
Marque : Everest Biotech
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Téléphone : +1 850 650 7790
| Code | Name | Applications | Availability | Size | Tested species | Grade |
|---|---|---|---|---|---|---|
| EB06694 | Goat Anti-CFTR Antibody | Pep-ELISA, IHC | 100µg specific antibody in 200µl | Human |
EB06694 (2.5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06694 (2.5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Target Protein
Principal Names: CFTR, CF, MRP7, ABC35, ABCC7, TNR-CFTR, dJ760C5.1, cystic fibrosis transmembrane conductance regulator, ATP-binding cassette (sub-family C, member 7), CFTR/MRP, ATP-binding cassette, sub-family C member 7Official Symbol: CFTR
Accession Number(s): NP_000483.3
Human GeneID(s): 1080
Non-Human GeneID(s): 12638 (mouse), 24255 (rat)
Immunogen
Peptide with sequence QRSPLEKASVVSK-C, from the N Terminus of the protein sequence according to NP_000483.3.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Kidney shows membranous staining in DCT cells. Paraffin embedded Human Prostate. Recommended concentration: 2.5µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat, Dog, Cow
- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol