Goat Anti-Mouse IgG2a Secondary Antibody (TR)
Référence LS-C209477-1
Conditionnement : 1mg
Marque : LSBio
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Téléphone : +1 850 650 7790
Overview
Antibody:
Mouse IgG2a Goat anti-Mouse Polyclonal (TR) Antibody
Application:
IHC, IF, WB, DB, FLISA
Reactivity:
Mouse
Format:
Texas Red, Unmodified
Specifications
Description
Mouse IgG2a antibody LS-C209477 is a TR-conjugated goat polyclonal antibody. Validated for DB, FLISA, IF, IHC and WB.
Target
Mouse IgG2a
Host
Goat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Texas Red
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Mouse IgG2a heavy chain.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse heavy or light chain isotypes. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
Applications
- IHC (1:1000 - 1:5000)
- Immunofluorescence (1:1000 - 1:5000)
- Western blot (1:4000 - 1:20000)
- Dot Blot
- Fluorophore-Linked Immunosorbent Assay (1:10000 - 1:50000)
Usage
Anti-Mouse IgG2a Texas Red alpha Conjugated antibodies are suitable for ELISA, Immunohistochemistry, western blotting, immunofluorescence microscopy, fluorescence based plate assays (ELISA) and fluorescent western blotting as well as other anti IgG2a antibody based assays. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
tech@clinisciences.com