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> Goat Human IgD (Fc specific), conjugated with Horseradish peroxidase Antibody

Goat Human IgD (Fc specific), conjugated with Horseradish peroxidase Antibody

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Référence orb21815-1ml

Conditionnement : 1ml

Marque : Biorbyt


Goat Human IgD (Fc specific), conjugated with Horseradish peroxidase Antibody

SKU: orb21815
This product can be custom made

Description

Horseradish peroxidase-conjugated IgG fraction of polyclonal goat antiserum to human IgD, Fc specific. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demon- strate circulating IgD antibodies in serodiagnostic microbiology and autoimmune diseases; in non- isotopic assay methodology (e.g. ELISA) to measure IgD in human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions may vary widely: for histochemical and cytochemical use they are usually between 1:50 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:10,000.

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

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Goat Human IgD (Fc specific), conjugated with Horseradish peroxidase Antibody
ReactivityHuman

Key Properties

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Antibody TypeSecondary Antibody
HostGoat
ClonalityPolyclonal
ConjugationHRP

Storage & Handling

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StorageThe lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Form/AppearanceHorseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Protocol Information