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> Goat Mouse IgG2 (subclass specific), conjugated with TRITC Antibody

Goat Mouse IgG2 (subclass specific), conjugated with TRITC Antibody

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Référence orb21382-1ml

Conditionnement : 1ml

Marque : Biorbyt


Goat Mouse IgG2 (subclass specific), conjugated with TRITC Antibody

SKU: orb21382
This product can be custom made

Description

Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to Mouse IgG2, subclass specific. In immunocytochemical and immunohistochemical single and double staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; as indicating second antibody conjugate to analyze or separate human cells after reacting with monoclonal mouse antibodies in immunofluorescence microscopy, fluorescence activated cell sorting and flow cytometry; to identify and measure IgG2 in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40, depending on the method used.

Research Area

Cell Biology

Images & Validation

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Goat Mouse IgG2 (subclass specific), conjugated with TRITC Antibody
ReactivityMouse

Key Properties

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Antibody TypeSecondary Antibody
HostGoat
ClonalityPolyclonal
ConjugationTRITC

Storage & Handling

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StorageThe lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Form/AppearanceTRITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Expiration Date12 mo