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> Goat Rat IgG1 IgG2a IgG2b IgG2c (heavy and light chains), conjugated with FITC Antibody

Goat Rat IgG1 IgG2a IgG2b IgG2c (heavy and light chains), conjugated with FITC Antibody

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Référence orb21479-2ml

Conditionnement : 2ml

Marque : Biorbyt


Goat Rat IgG1 IgG2a IgG2b IgG2c (heavy and light chains), conjugated with FITC Antibody

SKU: orb21479
This product can be custom made

Description

Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to rat IgG, heavy and light chains. To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immuno- fluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

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Goat Rat IgG1 IgG2a IgG2b IgG2c (heavy and light chains), conjugated with FITC Antibody
ReactivityRat

Key Properties

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Antibody TypeSecondary Antibody
HostGoat
ClonalityPolyclonal
ConjugationFITC

Storage & Handling

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StorageThe lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Form/AppearanceFITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only
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Protocol Information