Griffonia (Bandeiraea) simplicifolia GS I (FITC)

Référence G8967-20-2mg

Conditionnement : 2mg

Marque : US Biological



G8967-20 Griffonia (Bandeiraea) simplicifolia GS I (FITC)

Grade
Purified
Shipping Temp
Blue Ice
Storage Temp
-20°C

Pure Griffonia simplicifolia lectin (GS -I), conjugated to FITC

FITC/Protein Ratio: 2.15

Carbohydrate Specificity:
Melibiose, a-D-Galactose

Inhibitory Carbohydrate:
a-Galactose

Activity:
20-30ug/ml is required to agglutinate fresh type B blood cells. Lectin activity
against all blood types increases after neuraminidase treatment of the cells.

Note:
Calcium is required for binding. 0.5mM calcium is the maximum concentration in buffer that will not form a white precipitate.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

Applications
Purity: Purified by gel filtration performed after conjugation to remove free FITC. |Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS, pH 7.3, 0.5mM calcium chloride, 0.05% sodium azide. Labeled with FITC.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in PBS, pH 7.3, 0.5mM calcium chloride, 0.05% sodium azide. Labeled with FITC.
Purity
Purified by gel filtration performed after conjugation to remove free FITC.
References
1. Shankar Iyer, P.N.,et.al. (1976) Arch.Biochem.Biophys. 177,330. 2. Judd,W.J., et.al. (1977) Vox Sang, 33, 246. 3. Goldstein, I.J., et.al. (1978) Adv.Ca rbohydr.Chem. 35,127.