Griffonia simplicifolia Lectin I Isolectin B4 (Bandeiraea simplicifolia) (GSL I–isolectin B4, GS I-B4)

GSL I is a family of glycoproteins with molecular weights of ~114kD. There are two types of subunits, termed A and B , with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A -rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the B -rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.

GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons.

Inhibiting Sugar:
500mM galactose or 100mM raffinose

Applications:
G8967-93 will agglutinate blood group B glutaraldehyde-fixed human red blood cells at a concentration of 2ug/ml. No agglutination of blood group A, glutaraldehyde-fixed, human red blood cells occurred at concentrations up to 0.1mg/ml.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.