Histone H3 pS10/pT11 antibody
Référence orb420402-50ug
Conditionnement : 50ug
Marque : Biorbyt
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Histone H3 pS10/pT11 antibody
Catalog Number: orb420402
| Catalog Number | orb420402 |
|---|---|
| Category | Antibodies |
| Description | Histone H3 pS10/pT11 antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ChIP, IF, IHC, WB |
| Reactivity | C. elegans, Human |
| Isotype | IgG |
| Immunogen | Histone H3 [p Ser10, p Thr11] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phosphorylated peptide surrounding Serine 10 and Threonine 11 of human Histone H3.2. |
| Concentration | 1.2 mg/ml |
| Dilution range | ChIP: 2-5µg/million cells, IHC: 1:200, IF: 1:200, WB: 1:500 |
| Form/Appearance | Liquid (sterile filtered) |
| Purity | Anti-Histone H3 [p Ser10, p Thr11] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined. |
| Conjugation | Unconjugated |
| UniProt ID | Q71DI3 |
| NCBI | NP_001005464 |
| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | rabbit anti-Histone H3 pS10 pT11 antibody, H3.3B, |
| Note | For research use only |
| Application notes | Anti-Histone H3 [p Ser10, p Thr11] antibody is tested for Western Blot and Immunofluorescence. This antibody is useful for Dot Blot and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals. |
| Expiration Date | 12 months from date of receipt. |