Orb623182-1l | HRP Conjugate Stabilizer, Mammalian, 1X Clinisciences

HRP Conjugate Stabilizer, Mammalian, 1X

SKU: orb623182

Description

Preserve activity of HRP conjugates and reduce background signal in immunoassays using HRP detection systems. HRP Conjugate Stabilizer is used to preserve concentrated stock conjugates, reconstitute lyophilized conjugates, and dilute antibody-HRP conjugates to their useful working titer in ELISAs and immunology-based techniques.

Images & Validation

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Application Notes	1. For in vitro apoptosis detection, culture your cells to a concentration of 2-5 x 105 cells/mL. 2. Prepare experimental and control populations; concentrate cells to 5 x 105-106 cells/mL in 300 µL samples. 3. Reconstitute the FAM-OPH reagent with 50 µL DMSO to form the 150X stock concentrate (may be frozen for future use). 4. Dilute the FAM-OPH stock concentrate with 200 µL PBS to form the 30X working solution. 5. Add FAM-OPH working solution directly to samples and controls at a ratio of 1:30. For example, add 10 µL of working solution to a 300 µL aliquot of suspension cells. 6. Incubate ~1 hour. 7. Wash and spin cells two or three times. 8. If desired, label cells with Hoechst 33342 nuclear stain, DAPI nuclear stain, or other compatible fluorescent markers. 9. If desired, fix cells with formalin-based agents. 10. Analyze data using a fluorescence microscope, plate reader, or flow cytometer.

Application Notes

1. For in vitro apoptosis detection, culture your cells to a concentration of 2-5 x 105 cells/mL. 2. Prepare experimental and control populations; concentrate cells to 5 x 105-106 cells/mL in 300 µL samples. 3. Reconstitute the FAM-OPH reagent with 50 µL DMSO to form the 150X stock concentrate (may be frozen for future use). 4. Dilute the FAM-OPH stock concentrate with 200 µL PBS to form the 30X working solution. 5. Add FAM-OPH working solution directly to samples and controls at a ratio of 1:30. For example, add 10 µL of working solution to a 300 µL aliquot of suspension cells. 6. Incubate ~1 hour. 7. Wash and spin cells two or three times. 8. If desired, label cells with Hoechst 33342 nuclear stain, DAPI nuclear stain, or other compatible fluorescent markers. 9. If desired, fix cells with formalin-based agents. 10. Analyze data using a fluorescence microscope, plate reader, or flow cytometer.

Key Properties

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Storage & Handling

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Storage	2-8°C
Concentration	1 X
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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HRP Conjugate Stabilizer, Mammalian, 1X

Référence orb623182-1l

Marque : Biorbyt

HRP Conjugate Stabilizer, Mammalian, 1X

Description

Images & Validation

Key Properties

Storage & Handling

Neptune HRP Conjugate Stabilizer, Non-Mammalian 1X [orb623181]

Neptune HRP Conjugate Stabilizer, Non-Mammalian 1X [orb699917]

HRP Conjugate Stabilizer, Mammalian, 1X [orb699918]

HRP Conjugate Stabilizer, Mammalian, 1X [orb1821198]

Neptune HRP Conjugate Stabilizer, Non-Mammalian 1X [orb1821199]

Protocol Information