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> HSP90 beta Antibody

HSP90 beta Antibody

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Référence ASM10051-A655-100

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  • IHC - HSP90 beta Antibody ASM10051
    Immunohistochemistry analysis using Mouse Anti-Hsp90 beta Monoclonal Antibody, Clone K3701 (ASM10051). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 beta Monoclonal Antibody (ASM10051) at 1:3000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.
  • IHC - HSP90 beta Antibody ASM10051
    Immunohistochemistry analysis using Mouse Anti-Hsp90 beta Monoclonal Antibody, Clone K3701 (ASM10051). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 beta Monoclonal Antibody (ASM10051) at 1:3000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.
  • WB - HSP90 beta Antibody ASM10051
    Western Blot analysis of Human Cell lysates showing detection of Hsp90 beta protein using Mouse Anti-Hsp90 beta Monoclonal Antibody, Clone K3701 (ASM10051). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 beta Monoclonal Antibody (ASM10051) at 1.5 µg/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession P08238
Other Accession NP_031381.2
Host Mouse
Isotype IgM
Reactivity Human, Mouse
Clonality Monoclonal
Description Mouse Anti-Human HSP90 beta Monoclonal IgM
Target/Specificity Detects 90kDa. This is a beta specific product, does not cross-react with alpha isoforms.
Other Names HSP84 Antibody, HSP90B Antibody, HSPC2 Antibody, HSPCB Antibody, D6S182. FLJ26984 Antibody
Clone Names Hyb-K3701
Immunogen Recombinant human HSP90beta; Specificity mapped to amino acids 185-335
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml was sufficient for detection of HSP90β in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Cellular Localization Cytoplasm | Melanosome