Human Angiogenesis Array GS1

Référence GSH-ANG-1-4

Conditionnement : 64SampleKit

Marque : RayBiotech


Human Angiogenesis Array GS1

G-Series Human Angiogenesis Array 1 Kit. Semi-quantitative measurement of 10 human cytokines. Suitable for all liquid sample types.

Contact Us for Bulk Request

Product Description

Specifications

Size16 Sample Kit, 32 Sample Kit, 64 Sample Kit
SpeciesHuman
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-QuantitativeSemi-Quantitative
Number of Targets Detected10
Solid Support
Glass Slide
Design PrincipleSandwich-based
Method Of DetectionFluorescence Laser Scanner
Research AreaAngiogenesis, HER/ErbB Signaling, Vascular Biology
Shipping TypeBlue ice
Storage-20°C

Introduction

RayBio® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.

This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).

Product Features

  • High sensitivity (pg/mL to ng/mL levels)
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Test 16 samples on each slide
  • Data analysis software provides one-step computation

Target Names

Scroll over each target protein for more information
Angiogenin
  • Gene Symbol: ANG
  • Gene ID: 283
  • Accession #: P03950
  • ELISA Kit: RayBio Human Angiogenin ELISA
Angiopoietin-2
  • Gene Symbol: ANGPT2
  • Gene ID: 285
  • Accession #: O15123
  • ELISA Kit: RayBio Human ANGPT2 ELISA
EGF
  • Gene Symbol: EGF
  • Gene ID: 1950
  • Accession #: P01133
  • ELISA Kit: RayBio Human EGF ELISA
bFGF
  • Gene Symbol: FGF2
  • Gene ID: 2247
  • Accession #: P09038
  • ELISA Kit: RayBio Human bFGF ELISA
HB-EGF
  • Gene Symbol: HBEGF
  • Gene ID: 1839
  • Accession #: Q99075
  • ELISA Kit: RayBio Human HB-EGF ELISA
HGF
  • Gene Symbol: HGF
  • Gene ID: 3082
  • Accession #: P14210
  • ELISA Kit: RayBio Human HGF ELISA
Leptin
  • Gene Symbol: LEP
  • Gene ID: 3952
  • Accession #: P41159
  • ELISA Kit: RayBio Human Leptin ELISA
PDGF-BB
  • Gene Symbol: PDGFB
  • Gene ID: 5155
  • Accession #: P01127
  • ELISA Kit: RayBio Human PDGF-BB ELISA
PLGF
  • Gene Symbol: PGF
  • Gene ID: 5228
  • Accession #: P49763
  • ELISA Kit: RayBio Human PLGF ELISA
VEGF-A
  • Gene Symbol: VEGFA
  • Gene ID: 7422
  • Accession #: P15692
  • ELISA Kit: RayBio Human VEGF-A ELISA

Application Notes

Suggested Application
  • Multiplexed Protein Detection
  • Detection of Relative Protein Expression
  • Detecting Patterns of Cytokine Expression
  • Biomarker Screening
  • Identifying Key Factors
  • Confirming a Biological Process
Kit Components
  • Antibody Array Slide/s
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with samples
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

GAL (GAL-QAH-ANG-1.gal)
Excel (GSH-ANG-1_Analysis_Tool.xlsx)

citations

  • Manipulating bioactivities of endothelial progenitor cell-derived exosomes for promoting angiogenesis in ischemic vascular diseases

    Species:
    Human
    Sample Type:
    HUVECs
    Year:
    2023
    Journal Name:
    Nano Today
  • Plasma cytokines for predicting diabetic retinopathy among type 2 diabetic patients via machine learning algorithms

    Species:
    Human
    Sample Type:
    Plasma
    Year:
    2020
    Journal Name:
    Aging
    PubMed ID:
    33323553