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> Human Estradiol,E2 ELISA Kit

Human Estradiol,E2 ELISA Kit

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Référence CSB-E05108h-96T

Conditionnement : 96T

Marque : Cusabio


CSB-E05108h
Size 96T,5×96T,10×96T

Product Details

Associated names
N/A
Abbreviation
E2
Species
Homo sapiens (Human)
Sample Types
serum, plasma.
Detection Range
50 pg/ml-1200 pg/ml.
Sensitivity
40 pg/ml.
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

0

1.952

1.834

1.893

50

1.493

1.567

1.530

120

1.045

1.028

1.037

250

0.679

0.629

0.654

500

0.419

0.421

0.420

1200

0.234

0.243

0.239

Materials provided
    • A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit E2 antibody.
    • Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
    • Anti-mouse E2 Antibody 1 x 6 ml -- Act as the detection antibody.
    • HRP-conjugate 1 x 6 ml -- Bind to the E2 antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
    • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
    • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
    • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
    • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual
Materials not provided
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution
ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description

The human Estradiol (E2) ELISA kit is a powerful tool for quantitative measurement of estradiol levels in human serum and plasma samples, with a detection range of 50 pg/ml to 1200 pg/ml and a sensitivity of 40 pg/ml. The assay principle is competitive, meaning that two antibodies competitively bind to the estradiol in the sample. The detection is read at a wavelength of 450 nm. The assay time typically ranges from 1 to 5 hours, requiring a sample volume of 50-100 µl. The kit's performance has been validated in multiple studies found in more than 18 publications, ensuring its accuracy and r...

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