General Info
| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.94ng/mL |
| Detection Limit: | 1.56~100ng/mL |
| Short Description: | This human FABP1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
Information
| Gene Symbol: | FABP1 |
| Gene ID: | 2168 |
| Uniprot ID: | FABPL_HUMAN |
| Specificity: | This kit recognizes Human FABP1 in samples. No significant cross-reactivity or interference between Human FABP1 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Function | Plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes. Binds cholesterol. Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport. |
| Protein Name | Fatty Acid-Binding Protein - LiverFatty Acid-Binding Protein 1Liver-Type Fatty Acid-Binding ProteinL-Fabp |
| Database Links | Reactome: R-HSA-163560Reactome: R-HSA-189483Reactome: R-HSA-1989781Reactome: R-HSA-400206Reactome: R-HSA-9707564 |
| Cellular Localisation | Cytoplasm |
| Alternative CLIA Names | Fatty Acid-Binding Protein - Liver CLIA kitFatty Acid-Binding Protein 1 CLIA kitLiver-Type Fatty Acid-Binding Protein CLIA kitL-Fabp CLIA kitFABP1 CLIA kitFABPL CLIA kit |
| Specificity | This kit recognizes Human FABP1 in samples. No significant cross-reactivity or interference between Human FABP1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 100-112 | 106 |
| EDTA plasma(n=8) | 97-110 | 104 |
| Cell culture media(n=8) | 91-106 | 97 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 5.20 | 14.55 | 47.39 | 4.71 | 15.70 | 43.79 |
| Standard deviation | 0.57 | 1.14 | 4.74 | 0.50 | 1.86 | 4.67 |
| CV (%) | 10.96 | 7.84 | 10.00 | 10.62 | 11.85 | 10.66 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance