Human FGF4 (Fibroblast growth factor 4) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	FGF4
Gene ID:	2249
Uniprot ID:	FGF4_HUMAN
Specificity:	This kit recognizes Human FGF4 in samples. No significant cross-reactivity or interference between Human FGF4 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Function	Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Required for normal limb and cardiac valve development during embryogenesis. May play a role in embryonic molar tooth bud development via inducing the expression of MSX1, MSX2 and MSX1-mediated expression of SDC1 in dental mesenchyme cells.
Protein Name	Fibroblast Growth Factor 4 Fgf-4 Heparin Secretory-Transforming Protein 1 Hst Hst-1 Hstf-1 Heparin-Binding Growth Factor 4 Hbgf-4 Transforming Protein Ks3
Database Links	Reactome: R-HSA-109704 Reactome: R-HSA-1257604 Reactome: R-HSA-1839122 Reactome: R-HSA-1839130 Reactome: R-HSA-190322 Reactome: R-HSA-190372 Reactome: R-HSA-190373 Reactome: R-HSA-190375 Reactome: R-HSA-2033519 Reactome: R-HSA-2219530 Reactome: R-HSA-5654219 Reactome: R-HSA-5654221 Reactome: R-HSA-5654227 Reactome: R-HSA-5654228 Reactome: R-HSA-5654687 Reactome: R-HSA-5654688 Reactome: R-HSA-5654689 Reactome: R-HSA-5654693 Reactome: R-HSA-5654695 Reactome: R-HSA-5654699 Reactome: R-HSA-5654700 Reactome: R-HSA-5654704 Reactome: R-HSA-5654706 Reactome: R-HSA-5654710 Reactome: R-HSA-5654712 Reactome: R-HSA-5654719 Reactome: R-HSA-5654720 Reactome: R-HSA-5654726 Reactome: R-HSA-5654727 Reactome: R-HSA-5654732 Reactome: R-HSA-5654733 Reactome: R-HSA-5655253 Reactome: R-HSA-5655302 Reactome: R-HSA-5655332 Reactome: R-HSA-5658623 Reactome: R-HSA-5673001 Reactome: R-HSA-6811558
Cellular Localisation	Secreted
Alternative CLIA Names	Fibroblast Growth Factor 4 CLIA kit Fgf-4 CLIA kit Heparin Secretory-Transforming Protein 1 CLIA kit Hst CLIA kit Hst-1 CLIA kit Hstf-1 CLIA kit Heparin-Binding Growth Factor 4 CLIA kit Hbgf-4 CLIA kit Transforming Protein Ks3 CLIA kit FGF4 CLIA kit HST CLIA kit HSTF1 CLIA kit KS3 CLIA kit
Specificity	This kit recognizes Human FGF4 in samples. No significant cross-reactivity or interference between Human FGF4 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-105	98
EDTA plasma(n=8)	90-106	97
Cell culture media(n=8)	85-98	90

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	51.21	92.16	471.86	50.19	98.25	495.90
Standard deviation	6.24	9.43	36.05	4.20	11.55	54.35
CV (%)	12.19	10.23	7.64	8.37	11.76	10.96

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance