Human FGF6 (Fibroblast growth factor 6) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	FGF6
Gene ID:	2251
Uniprot ID:	FGF6_HUMAN
Specificity:	This kit recognizes Human FGF6 in samples. No significant cross-reactivity or interference between Human FGF6 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Leukemia cell lines with platelet/ megakaryocytic differentiation potential.
Function	Plays an important role in the regulation of cell proliferation, cell differentiation, angiogenesis and myogenesis, and is required for normal muscle regeneration.
Protein Name	Fibroblast Growth Factor 6 Fgf-6 Heparin Secretory-Transforming Protein 2 Hst-2 Hstf-2 Heparin-Binding Growth Factor 6 Hbgf-6
Database Links	Reactome: R-HSA-109704 Reactome: R-HSA-1257604 Reactome: R-HSA-1839122 Reactome: R-HSA-190322 Reactome: R-HSA-190373 Reactome: R-HSA-190375 Reactome: R-HSA-2033519 Reactome: R-HSA-2219530 Reactome: R-HSA-5654219 Reactome: R-HSA-5654221 Reactome: R-HSA-5654228 Reactome: R-HSA-5654687 Reactome: R-HSA-5654688 Reactome: R-HSA-5654689 Reactome: R-HSA-5654693 Reactome: R-HSA-5654695 Reactome: R-HSA-5654699 Reactome: R-HSA-5654700 Reactome: R-HSA-5654712 Reactome: R-HSA-5654719 Reactome: R-HSA-5654720 Reactome: R-HSA-5654726 Reactome: R-HSA-5654727 Reactome: R-HSA-5654733 Reactome: R-HSA-5655253 Reactome: R-HSA-5655302 Reactome: R-HSA-5673001 Reactome: R-HSA-6811558
Cellular Localisation	Secreted Extracellular Space
Alternative CLIA Names	Fibroblast Growth Factor 6 CLIA kit Fgf-6 CLIA kit Heparin Secretory-Transforming Protein 2 CLIA kit Hst-2 CLIA kit Hstf-2 CLIA kit Heparin-Binding Growth Factor 6 CLIA kit Hbgf-6 CLIA kit FGF6 CLIA kit HST2 CLIA kit HSTF2 CLIA kit
Specificity	This kit recognizes Human FGF6 in samples. No significant cross-reactivity or interference between Human FGF6 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-99	91
EDTA plasma(n=8)	95-111	102
Cell culture media(n=8)	101-118	108

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	102.84	241.06	994.17	99.62	238.65	901.77
Standard deviation	9.93	18.10	102.80	10.95	16.87	92.34
CV (%)	9.66	7.51	10.34	10.99	7.07	10.24

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance