Human IGF2R (Cation-independent mannose-6-phosphate receptor) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	IGF2R
Gene ID:	3482
Uniprot ID:	MPRI_HUMAN
Specificity:	This kit recognizes Human IGF2R in samples. No significant cross-reactivity or interference between Human IGF2R and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Palmitoylated. Undergoes cysteine S-palmitoylation which promotes interaction with the retromer cargo-selective complex which mediates its retrograde trafficking to the Golgi apparatus.
Function	Mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex. The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation by binding DPP4.
Protein Name	Cation-Independent Mannose-6-Phosphate Receptor Ci Man-6-P Receptor Ci-Mpr M6pr 300 Kda Mannose 6-Phosphate Receptor Mpr 300 Insulin-Like Growth Factor 2 Receptor Insulin-Like Growth Factor Ii Receptor Igf-Ii Receptor M6p/Igf2 Receptor M6p/Igf2r Cd Antigen Cd222
Database Links	Reactome: R-HSA-432722 Reactome: R-HSA-6798695 Reactome: R-HSA-6811440 Reactome: R-HSA-8856825 Reactome: R-HSA-8856828
Cellular Localisation	Golgi Apparatus Membrane Single-Pass Type I Membrane Protein Endosome Membrane Mainly Localized In The Golgi At Steady State And Not Detectable In Lysosome Colocalized With Dpp4 In Internalized Cytoplasmic Vesicles Adjacent To The Cell Surface
Alternative CLIA Names	Cation-Independent Mannose-6-Phosphate Receptor CLIA kit Ci Man-6-P Receptor CLIA kit Ci-Mpr CLIA kit M6pr CLIA kit 300 Kda Mannose 6-Phosphate Receptor CLIA kit Mpr 300 CLIA kit Insulin-Like Growth Factor 2 Receptor CLIA kit Insulin-Like Growth Factor Ii Receptor CLIA kit Igf-Ii Receptor CLIA kit M6p/Igf2 Receptor CLIA kit M6p/Igf2r CLIA kit Cd Antigen Cd222 CLIA kit IGF2R CLIA kit MPRI CLIA kit
Specificity	This kit recognizes Human IGF2R in samples. No significant cross-reactivity or interference between Human IGF2R and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	95-108	101
EDTA plasma(n=8)	94-106	99
Cell culture media(n=8)	89-104	95

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	419.94	789.36	3128.14	412.07	809.34	3137.23
Standard deviation	43.38	94.72	253.07	43.47	82.80	209.88
CV (%)	10.33	12.00	8.09	10.55	10.23	6.69

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance