RayBio® Human IL-1 alpha (IL-1 F1) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
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Human IL-1 alpha ELISA Kit
Human IL-1 alpha ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 0.5 pg/ml Need more sensitivity? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 0.5 pg/ml - 300 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P01583 |
| Gene Id | 3552 |
| Gene Symbols | IL1A, IL1F1 |
| Protein Name / Synonyms | Interleukin-1 alpha (IL-1 alpha) (Hematopoietin-1) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cancer, Inflammation, Antibody Drug Development, Protein Therapeutics |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
| Sample type | Cell Culture Supernatants / Plasma / Serum, Cell and Tissue Lysates |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human IL-1 alpha ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified IL-1 alpha (IL-1F1 / IL1A), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
HPV16-Expressing Tumors Release Multiple IL1 Ligands to Orchestrate Systemic Immunosuppression Whose Disruption Enables Efficacy of a Therapeutic Vaccine
- Species:
- Human
- Sample Type:
- Serum (cervical cancer patients vs normal donors)
- Year:
- 2025
- Journal Name:
- Cancer Discovery
- PubMed ID:
- 40287949
Substrate topographies modulate the secretory activity of human bone marrow mesenchymal stem cells
- Species:
- Human
- Sample Type:
- bone marrow-derived Mesenchymal stem cell
- Year:
- 2023
- Journal Name:
- Stem Cell Research & Therapy
How much obesity and diabetes do impair male fertility?
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Reproductive Biology and Endocrinology
Inhibition of human macrophage activation via pregnane neurosteroid interactions with toll-like receptors: Sex differences and structural requirements
- Species:
- Human
- Sample Type:
- human monocyte-derived macrophages (hMDM)
- Year:
- 2022
- Journal Name:
- Frontiers in immunology
Serum Levels of IL-1 RA Increase with Obesity and Type 2 Diabetes in Relation to Adipose Tissue Dysfunction and are Reduced After Bariatric Surgery in Parallel to Adiposity
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Journal of inflammation research
LncRNA Sirt1-AS upregulates Sirt1 to attenuate aging related deep venous thrombosis
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant; Whole Blood (blood from mice)
- Year:
- 2021
- Journal Name:
- Aging
- PubMed ID:
- 33638947
Urine protein biomarkers of bladder cancer arising from 16-plex antibody-based screens
- Species:
- Human
- Sample Type:
- Urine (bladder cancer patients and controls)
- Year:
- 2021
- Journal Name:
- Oncotarget
- PubMed ID:
- 33889301
Low vitamin D status is associated with inflammatory response in older patients with cerebral small vessel disease
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Journal of Neuroimmunology
Immunomodulatory Activities of Medicinal Mushroom Grifola frondosa Extract and Its Bioactive Constituent
- Species:
- Human
- Sample Type:
- Conditioned Media (THP-1 promonocytic cell line, treated and control)
- Year:
- 2013
- Journal Name:
- The American Journal of Chinese Medicine
Bystander effect in tumor cells produced by Iodine-125 labeled human lymphocytes
- Species:
- Human
- Sample Type:
- Conditioned Media (Colon adenocarcinoma LS174T cells co-culutred with peropheral blood lymphocytes )
- Year:
- 2012
- Journal Name:
- International Journal of Radiation Oncology • Biology • Physics
- PubMed ID:
- 22712706