RayBio® Human IL-1 alpha (IL-1 F1) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
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Human IL-1 alpha ELISA Kit
Human IL-1 alpha ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 0.5 pg/ml Need more sensitivity? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 0.5 pg/ml - 300 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P01583 |
| Gene Id | 3552 |
| Gene Symbols | IL1A, IL1F1 |
| Protein Name / Synonyms | Interleukin-1 alpha (IL-1 alpha) (Hematopoietin-1) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cancer, Inflammation, Antibody Drug Development, Protein Therapeutics |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
| Sample type | Cell Culture Supernatants / Plasma / Serum, Cell and Tissue Lysates |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
How much obesity and diabetes do impair male fertility?
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Reproductive Biology and Endocrinology
Low vitamin D status is associated with inflammatory response in older patients with cerebral small vessel disease
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Journal of Neuroimmunology
Substrate topographies modulate the secretory activity of human bone marrow mesenchymal stem cells
- Species:
- Human
- Sample Type:
- bone marrow-derived Mesenchymal stem cell
- Year:
- 2023
- Journal Name:
- Stem Cell Research & Therapy
Inhibition of human macrophage activation via pregnane neurosteroid interactions with toll-like receptors: Sex differences and structural requirements
- Species:
- Human
- Sample Type:
- human monocyte-derived macrophages (hMDM)
- Year:
- 2022
- Journal Name:
- Frontiers in immunology
Serum Levels of IL-1 RA Increase with Obesity and Type 2 Diabetes in Relation to Adipose Tissue Dysfunction and are Reduced After Bariatric Surgery in Parallel to Adiposity
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Journal of inflammation research
Functional polymorphism rs3783553 in the 3'-untranslated region of IL-1A increased the risk of ischemic stroke: A case-control study
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2017
- Journal Name:
- Medicine
Effect of cadmium on the expression levels of interleukin-1 and interleukin-10 cytokines in human lung cells
- Species:
- Human
- Sample Type:
- Conditioned Media (Cadmium treated MRC-9 lung cells)
- Year:
- 2015
- Journal Name:
- Molecular Medicine Reports
- PubMed ID:
- 26397147
Correlation Between Interleukins 1 ,6 and Echocardiographic Results in Patients with Heart Failure
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2014
Effect of DMPA on IL-1 Alpha in Serum of Women with Endometrioma.
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2013
- Journal Name:
- Thai Journal of Obstetrics and Gynaecology
Immunomodulatory Activities of Medicinal Mushroom Grifola frondosa Extract and Its Bioactive Constituent
- Species:
- Human
- Sample Type:
- Conditioned Media (THP-1 promonocytic cell line, treated and control)
- Year:
- 2013
- Journal Name:
- The American Journal of Chinese Medicine