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> Human Kappa Light Chain(Kap-56), CF740 conjugate, 0.1mg/mL

Human Kappa Light Chain(Kap-56), CF740 conjugate, 0.1mg/mL

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Référence BNC740859-500

Conditionnement : 500uL

Marque : Biotium

Human Kappa Light Chain(Kap-56), CF740 conjugate, 0.1mg/mL

Human Kappa Light Chain Monoclonal Mouse Antibody (Kap-56)

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Monoclonal anti Kappa Light Chain (Kap 56)Formalin-fixed, paraffin-embedded human Tonsil stained with Kappa Light Chain Monoclonal Antibody (Kap-56).
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Product Description

This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email [email protected] to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 0859, Anti-Human Kappa Light Chain (Kap-56) 

Antibody # prefixConjugationEx/Em (nm)Laser lineDetection channelDye Features
BNC04CF®405S404/431405DAPI (microscopy), AF405CF®405S Features
BNC88CF®488A490/515488GFP, FITCCF®488A Features
BNC68CF®568562/583532, 561RFP, TRITCCF®568 Features
BNC94CF®594593/614561Texas Red®CF®594 Features
BNC40CF®640R642/662633-640Cy®5CF®640R Features
BNC47CF®647650/665633-640Cy®5CF®647 Features
BNC74CF®740742/767633-685775/50CF®740 Features
BNCBBiotinN/AN/AN/A
BNUBPurifiedN/AN/AN/A
BNUMPurified,
BSA-free		N/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.
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Primary & Secondary Antibody Conjugates

Product Attributes

Antibody number
#0859
Antibody reactivity (target)
Human Kappa Light Chain
Antibody type
Primary, Anti-Human Immunoglobulin
Host species
Mouse
Clonality
Monoclonal
Clone
Kap-56
Isotype
IgG1, kappa
Molecular weight
~22.5 kDa
Synonyms
HCAK1; Ig Kappa Chain C Region; IGKC; Immunoglobulin KM
Human gene symbol
IGKC
Entrez gene ID
3514
SwissProt
P01601 & P01834
Unigene
449609
Immunogen
Human lymphocytes stimulated
Antibody target cellular localization
Plasma membrane, Secreted (extracellular)
Verified antibody applications
IHC (FFPE) (verified)
Species reactivity
Human
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunohistology formalin-fixed 0.5-1.0 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Western blotting 0.5-1.0 ug/mL, Flow Cytometry 0.5-2.0 ug/million cells in 0.1 mL, Optimal dilution for a specific application should be determined by user
Positive control
293T, Raji or hPBL cells. Tonsil or Spleen
Shipping condition
Room temperature
Storage Conditions
Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status
For research use only (RUO)
Antibody/conjugate formulation
Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Cell/tissue expression
B-cells
Antibody research areas
Cancer, Immunology, Inflammation
Product origin
Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Tumor expression
Leukemia/lymphoma

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