Human Krueppel-like factor 4, KLF4 ELISA Kit
Référence MBS928766-5x96
Conditionnement : 5x96-Strip-Wells
Marque : MyBiosource
Kruppel-Like Factor 4 (Gut) (KLF4) ELISA Kit for Human
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS928766Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KLF4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLF4 present is bound by the… More details
Product Overview
Online Datasheet in PDFProduct Name
Kruppel-like factor 4 (gut) (KLF4), ELISA Kit
Full Product Name
Human Krueppel-like factor 4, KLF4 ELISA Kit
Product Gene Name
KLF4 elisa kit
Product Synonym Names
Human Krueppel-like factor 4 (KLF4) ELISA kit; EZF; GKLF; endothelial Kruppel-like zinc finger protein; Kruppel-like factor 4 (gut)
Species Reactivity
Human
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates, cell lysates
Detection Range
18.75 pg/ml-1200 pg/ml
Sensitivity
< 4.6 pg/ml
Specificity
This assay has high sensitivity and excellent specificity for detection of human KLF4. No significant cross-reactivity or interference between human KLF4 and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Molecular Weight
54,671 Da
OMIM
602253
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of KLF4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for KLF4 purchase
MBS928766 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Kruppel-like factor 4 (gut) (KLF4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KLF4. The ELISA analytical biochemical technique of the MBS928766 kit is based on KLF4 antibody-KLF4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KLF4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KLF4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for KLF4. It may not necessarily be applicable to this product.
NCBI Official Full Name
Krueppel-like factor 4
NCBI Official Synonym Full Names
Kruppel-like factor 4 (gut)
NCBI Official Symbol
KLF4
NCBI Official Synonym Symbols
EZF; GKLF
UniProt Protein Name
Krueppel-like factor 4
UniProt Synonym Protein Names
Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor
Protein Family
Krueppel-like factor
UniProt Gene Name
KLF4
UniProt Synonym Gene Names
EZF; GKLF
UniProt Entry Name
KLF4_HUMAN
NCBI Protein Information
Krueppel-like factor 4; gut-enriched krueppel-like factor; epithelial zinc finger protein EZF; endothelial Kruppel-like zinc finger protein
UniProt Comments for KLF4
KLF4: Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. Interacts with POU5F1/OCT4 and SOX2. Interacts with MUC1 (via the C-terminal domain). Belongs to the krueppel C2H2-type zinc-finger protein family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; DNA-binding; C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 9q31
Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin
Molecular Function: protein binding; zinc ion binding; double-stranded DNA binding; phosphoinositide 3-kinase regulator activity; transcription factor activity
Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; post-embryonic camera-type eye development; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; negative regulation of interleukin-8 biosynthetic process; negative regulation of muscle hyperplasia; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; positive regulation of protein metabolic process; positive regulation of hemoglobin biosynthetic process; regulation of phosphoinositide 3-kinase activity; negative regulation of cell proliferation; post-embryonic hemopoiesis; epidermis morphogenesis; regulation of cell differentiation; negative regulation of protein kinase B signaling cascade; response to retinoic acid; negative regulation of heterotypic cell-cell adhesion; stem cell maintenance; positive regulation of telomerase activity; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; epidermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; mesodermal cell fate determination; negative regulation of phosphoinositide 3-kinase cascade
Show more Protein type: Transcription factor; DNA-binding; C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 9q31
Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin
Molecular Function: protein binding; zinc ion binding; double-stranded DNA binding; phosphoinositide 3-kinase regulator activity; transcription factor activity
Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; post-embryonic camera-type eye development; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; negative regulation of interleukin-8 biosynthetic process; negative regulation of muscle hyperplasia; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; positive regulation of protein metabolic process; positive regulation of hemoglobin biosynthetic process; regulation of phosphoinositide 3-kinase activity; negative regulation of cell proliferation; post-embryonic hemopoiesis; epidermis morphogenesis; regulation of cell differentiation; negative regulation of protein kinase B signaling cascade; response to retinoic acid; negative regulation of heterotypic cell-cell adhesion; stem cell maintenance; positive regulation of telomerase activity; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; epidermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; mesodermal cell fate determination; negative regulation of phosphoinositide 3-kinase cascade
Research Articles on KLF4
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with KLF4 elisa kit
| Reference Product | PubMed Publications |
|---|---|
| SP1 elisa kit | >32 publications with KLF4 and SP1 |
| KLF6 elisa kit | >11 publications with KLF4 and KLF6 |
| TP53 elisa kit | >8 publications with KLF4 and TP53 |
| HDAC5 elisa kit | >3 publications with KLF4 and HDAC5 |
| ELK1 elisa kit | >2 publications with KLF4 and ELK1 |
| CREBBP elisa kit | >1 publications with KLF4 and CREBBP |
Pathways associated with KLF4 elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Developmental Biology Pathway antibodies | Developmental Biology Pathway Diagram |
| Diabetes Pathways antibodies | Diabetes Pathways Diagram |
| Disease Pathway antibodies | Disease Pathway Diagram |
| Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway antibodies | Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway Diagram |
| Synthesis, Secretion, And Deacylation Of Ghrelin Pathway antibodies | Synthesis, Secretion, And Deacylation Of Ghrelin Pathway Diagram |
| Transcriptional Regulation Of White Adipocyte Differentiation Pathway antibodies | Transcriptional Regulation Of White Adipocyte Differentiation Pathway Diagram |
Diseases associated with KLF4 elisa kit
| Disease Name | Pubmed Publications |
|---|---|
| Neoplasms Antibodies | >175 publications with KLF4 and Neoplasms |
| Gastrointestinal Diseases Antibodies | >54 publications with KLF4 and Gastrointestinal Diseases |
| Skin Diseases Antibodies | >39 publications with KLF4 and Skin Diseases |
| Cardiovascular Diseases Antibodies | >32 publications with KLF4 and Cardiovascular Diseases |
| Inflammation Antibodies | >31 publications with KLF4 and Inflammation |
| Vascular Diseases Antibodies | >29 publications with KLF4 and Vascular Diseases |
| Nervous System Diseases Antibodies | >27 publications with KLF4 and Nervous System Diseases |
| Breast Neoplasms Antibodies | >20 publications with KLF4 and Breast Neoplasms |
| Adenocarcinoma Antibodies | >18 publications with KLF4 and Adenocarcinoma |
| Disease Models, Animal Antibodies | >18 publications with KLF4 and Disease Models, Animal |
Organs/Tissues associated with KLF4 elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Embryonic Tissue Antibodies | >118 publications with KLF4 and Embryonic Tissue |
| Skin Antibodies | >79 publications with KLF4 and Skin |
| Vascular Antibodies | >64 publications with KLF4 and Vascular |
| Heart Antibodies | >40 publications with KLF4 and Heart |
| Intestine Antibodies | >36 publications with KLF4 and Intestine |
| Brain Antibodies | >32 publications with KLF4 and Brain |
| Liver Antibodies | >27 publications with KLF4 and Liver |
| Eye Antibodies | >22 publications with KLF4 and Eye |
| Mouth Antibodies | >17 publications with KLF4 and Mouth |
| Testis Antibodies | >17 publications with KLF4 and Testis |