RayBio® Human M-CSF ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human M-CSF ELISA Kit
Human M-CSF ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 5 pg/ml Need more sensitivity? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 5 pg/ml - 2000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P09603 |
| Gene Id | 1435 |
| Gene Symbols | CSF1 |
| Protein Name / Synonyms | Macrophage colony-stimulating factor 1 (CSF-1) (M-CSF) (MCSF) (Lanimostim) [Cleaved into: Processed macrophage colony-stimulating factor 1] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human M-CSF ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified M-CSF (CSF1), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Cytokine profile of cerebrospinal fluid in pediatric patients with metastatic medulloblastoma
- Species:
- Human
- Sample Type:
- Cerebrospinal Fluid
- Year:
- 2024
- Journal Name:
- Heliyon
- PubMed ID:
- 39524698
ASH1L in Hepatoma Cells and Hepatic Stellate Cells Promotes Fibrosis-Associated Hepatocellular Carcinoma by Modulating Tumor-Associated Macrophages
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (conditioned medium from the coculture system)
- Year:
- 2024
- Journal Name:
- Advanced science (Weinheim, Baden-Württemberg, Germany)
- PubMed ID:
- 39377228
Enteric Glia Modulate Macrophage Phenotype and Visceral Sensitivity following Inflammation
- Species:
- Human
- Sample Type:
- Conditioned Media (Primary Fibroblasts in cancer model)
- Year:
- 2020
- Journal Name:
- Cell Rep.
- PubMed ID:
- 30394883
Disruption of tumour-associated macrophage trafficking by the osteopontin-induced colony-stimulating factor-1 signalling sensitises hepatocellular carcinoma to anti-PD-L1 blockade
- Species:
- Human
- Sample Type:
- Conditioned Media (HepG2, Hep3B, MHCC97H, HCCLM3 Co Culture System)
- Year:
- 2019
- Journal Name:
- Gut
- PubMed ID:
- 30902885
Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (NSCLC cell culture supernatants)
- Year:
- 2019
- Journal Name:
- Redox Biology
- PubMed ID:
- 30769285
CD10 + GPR77 + Cancer-Associated Fibroblasts Promote Cancer Formation and Chemoresistance by Sustaining Cancer Stemness
- Species:
- Human
- Sample Type:
- Conditioned Media (Primary Fibroblasts in cancer model)
- Year:
- 2018
- Journal Name:
- Cell
- PubMed ID:
- 29395328
Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (48 h cell culture)
- Year:
- 2021
- Journal Name:
- Advanced science (Weinheim, Baden-Württemberg, Germany)
- PubMed ID:
- 33643790
A quantitative analysis of macrophage–colony-stimulating factor in peri-miniscrew implant crevicular fluid before and after orthodontic loading
- Species:
- Human
- Sample Type:
- peri-miniscrew implant crevicular fluid (PMICF)
- Year:
- 2022
- Journal Name:
- Angle Orthodontist
Cytokine Consistency Between Bone Marrow and Peripheral Blood in Patients With Philadelphia-Negative Myeloproliferative Neoplasms
- Species:
- Human
- Sample Type:
- Plasma (52 Ph-MPN patients, 20 healthy volunteers)
- Year:
- 2021
- Journal Name:
- Frontiers in Medicine
- PubMed ID:
- 34249954
Argonaute 2 Sustains the Gene Expression Program Driving Human Monocytic Differentiation of Acute Myeloid Leukemia Cells.
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2013
- Journal Name:
- Cell Death and Disease
