AAH-MAPK-1-8 | Human/Mouse MAPK Phosphorylation Array Clinisciences

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Human/Mouse MAPK Phosphorylation Array

Detects 17 phosphorylated proteins in human or mouse samples. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

Product Description

Specifications

Size	2 Sample Kit, 4 Sample Kit, 8 Sample Kit
Species	Human, Mouse
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	17
Recommended Applications	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Solid Support	Membrane
Design Principle	Sandwich-based
Method Of Detection	Chemiluminescence
Research Area	Post-Translational Modifications, Phosphorylation, HER/ErbB Signaling, IGF Signaling, JAK/STAT Signaling, MAPK Signaling, PI3K-AKT Signaling, NFkB Signaling, Wnt-beta-Catenin Signaling
Shipping Type	Blue ice
Storage	-20°C

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Akt (P-Ser473) (PKB (P-Ser473)) Gene Symbol: AKT1 PKB RAC Gene ID: 207 Accession #: P31749 ELISA Kit: RayBio Human/Mouse/Rat Phospho-Akt (S473) ELISA	CREB (P-Ser133) Gene Symbol: CREB1 Gene ID: 1385 Accession #: P16220 ELISA Kit: RayBio Human Phospho-CREB (S133) ELISA	ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187) Gene Symbol: MAPK1 ERK2 PRKM1 PRKM2 Gene ID: 5594 Accession #: P28482 ELISA Kit: RayBio Human/Mouse/Rat Phospho-Erk1 (T202/Y204) + Phospho-Erk2 (Y185/Y187) and Total Erk1/2 ELISA	GSK3a (P-Ser21) Gene Symbol: GSK3A Gene ID: 2931 Accession #: P49840 ELISA Kit: RayBio Human/Mouse/Rat Phospho-GSK3a (S21) ELISA	GSK3b (P-Ser9) Gene Symbol: GSK3B Gene ID: 2932 Accession #: P49841 ELISA Kit: RayBio Human Phospho-GSK3b (S9) ELISA
HSP27 (P-Ser82) Gene Symbol: HSPB1 HSP27 HSP28 Gene ID: 3315 Accession #: P04792 ELISA Kit: RayBio Human Phopspho-HSP27 (S82) ELISA	JNK (P-Thr183) Gene Symbol: MAPK8 JNK1 PRKM8 SAPK1 SAPK1C Gene ID: 5599 Accession #: P45983 ELISA Kit: RayBio Human/Mouse/Rat Phospho-JNK (T183/Y185) ELISA	MEK (P-Ser217/Ser221) Gene Symbol: MAP2K1 MEK1 PRKMK1 Gene ID: 5604 Accession #: Q02750 ELISA Kit: RayBio Human/Mouse/Rat Phospho-MEK1 (S217/S221) ELISA	MKK3 (P-Ser189) Gene Symbol: MAP2K3 MEK3 MKK3 PRKMK3 SKK2 Gene ID: 5606 Accession #: P46734 ELISA Kit: RayBio Human/Mouse/Rat Phospho-MKK3 (S189) ELISA	MKK6 (P-Ser207) Gene Symbol: MAP2K6 MEK6 MKK6 PRKMK6 SKK3 Gene ID: 5608 Accession #: P52564 ELISA Kit: RayBio Human/Mouse/Rat Phospho-MKK6 (S207) ELISA
MSK2 (P-Ser360) Gene Symbol: RPS6KA4 MSK2 Gene ID: 8986 Accession #: O75676 ELISA Kit: RayBio Human Phospho-MSK2 (S360) ELISA	mTOR (P-Ser2448) Gene Symbol: MTOR FRAP FRAP1 FRAP2 RAFT1 RAPT1 Gene ID: 2475 Accession #: P42345 ELISA Kit: RayBio Human Phospho-mTOR (S2448) ELISA	p38 (P-Thr180/Tyr182) Gene Symbol: MAPK14 CSBP CSBP1 CSBP2 CSPB1 MXI2 SAPK2A Gene ID: 1432 Accession #: Q16539 ELISA Kit: RayBio Human/Mouse Phospho-p38 alpha (T180/Y182) ELISA	P53 (P-Ser15) Gene Symbol: TP53 P53 Gene ID: 7157 Accession #: P04637 ELISA Kit: RayBio Human/Mouse/Rat Phospho-P53 (S15) ELISA	P70S6K (P-Thr421/Ser424) Gene Symbol: RPS6KB1 STK14A Gene ID: 6198 Accession #: P23443 ELISA Kit: RayBio Human/Mouse/Rat Phospho-P70S6K (T421/S424) ELISA
RSK1 (P-Ser380) Gene Symbol: RPS6KA1 MAPKAPK1A RSK1 Gene ID: 6195 Accession #: Q15418 ELISA Kit: RayBio Human Phosph-RSK1 (S380) ELISA	RSK2 (P-Ser386) Gene Symbol: RPS6KA3 ISPK1 MAPKAPK1B RSK2 Gene ID: 10432 Accession #: P51812

Kit Components

Human/Mouse MAPK Phosphorylation Antibody Array C1 Membranes
Blocking Buffer
Detection Antibody Cocktail
1,000X HRP-Anti-Rabbit-IgG Concentrate
20X Wash Buffer I Concentrate
20X Wash Buffer II Concentrate
2X Cell Lysis Buffer Concentrate
Detection Buffer C
Detection Buffer D
8-Well Incubation Tray w/ Lid
Protease Inhibitor Cocktail
100x Phosphatase Inhibitor Cocktail I
Phosphatase Inhibitor Cocktail II
Plastic Sheets
Array Map Template
Manual

Additional Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Assay Procedure Summary

Block membranes
Incubate with Sample
Incubate with Detection Antibody Cocktail
Incubate with HRP-Conjugated anti-IgG
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Typical Data

HeLa cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with 250 nM PMA for 20 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

HepG2 cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with 25 ng/mL of recombinant human IL-1β for 30 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

RayBiotech's MAPK Phosphorylation Antibody Array revealed that modulating miR-155-5p expression affects MAPK signaling in intervertebral disc cells under inflammatory conditions. Figure adapted from Cazzanelli et al., Cell Commun Signal, 2024.

RayBiotech's MAPK Phosphorylation Antibody Array revealed irisin stimulation activated MAPK signaling in mesenchymal stem cells. Figure adapted from Xue et al., BMC Biol, 2022.

RayBiotech's MAPK Phosphorylation Antibody Array (AAH-MAPK-1) was used to assess the effects of NADPH oxidase on MALP-2-dependent signaling in endothelial cells. Figure adapted from Schuett et al., FASEB J, 2017.

RayBiotech's MAPK Phosphorylation Antibody Array was used to assess the effects of fusaric acid on PI3K/Akt/mTOR signaling in the mouse brain tissue. Figure adapted from Dhani et al., Food Chem Toxicol, 2020.

RayBiotech's MAPK Phosphorylation Antibody Array revealed cytokine-induced MAPK signaling alterations in pancreatic islets. Figure adapted from Lee et al., FASEB J, 2024.

RayBiotech's MAPK Phosphorylation Antibody Array revealed MAPK pathway alterations in liver tissue following Mfap4 knockdown. Figure adapted from Iakovleva et al., npj Regenerative Medicine, 2023.

Storage/Stability

For best results, store the entire kit frozen at -20^°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20^°C and all other reagents undiluted at 4^°C for no more than 3 months.

documents

Datasheet (AAH-MAPK-1.pdf)

SDS (AAH-MAPK-1.pdf)

Excel (AAH-MAPK-1_Analysis_Tool.xls)

citations

The HRAS-binding C2 domain of PLCη2 suppresses tumor-like synoviocytes and experimental arthritis in rheumatoid arthritis
Year:
2025
Journal Name:
Experimental and Molecular Medicine
PubMed ID:
39894825
REG3A secreted by peritumoral acinar cells enhances pancreatic ductal adenocarcinoma progression via activation of EGFR signaling
Year:
2025
Journal Name:
Cell Communication and Signaling
PubMed ID:
39966859
Cannabinol modulates the endocannabinoid system and shows TRPV1-mediated anti-inflammatory properties in human keratinocytes
Year:
2025
Journal Name:
Biofactors
PubMed ID:
39275884
Chronic TNF exposure induces glucocorticoid-like immunosuppression in the alveolar macrophages of aged mice that enhances their susceptibility to pneumonia
Year:
2024
Journal Name:
Aging Cell
PubMed ID:
38459711
The role of miR-155-5p in inflammation and mechanical loading during intervertebral disc degeneration
Year:
2024
Journal Name:
Cell Communication and Signaling
PubMed ID:
39192354
Activation of MEK-ERK-c-MYC signaling pathway promotes splenic M2-like macrophage polarization to inhibit PHcH-liver cirrhosis
Year:
2024
Journal Name:
Frontiers in immunology
PubMed ID:
39620221
Effects of Metformin on JNK Signaling Pathway and PD-L1 Expression in Triple Negative Breast Cancer
Year:
2024
Journal Name:
Cancer management and research
PubMed ID:
38585433
Sodium butyrate prevents cytokine-induced β-cell dysfunction through restoration of stromal interaction molecule 1 expression and activation of store-operated calcium entry
Year:
2024
Journal Name:
FASEB J
PubMed ID:
39120544
Prolonged extracellular low sodium concentrations and subsequent their rapid correction modulate nitric oxide production dependent on NFAT5 in microglia
Year:
2024
Journal Name:
Free Radic Biol Med
PubMed ID:
39155026
Mfap4: a promising target for enhanced liver regeneration and chronic liver disease treatment
Year:
2023
Journal Name:
NPJ Regenerative medicine
PubMed ID:
37935709

Human/Mouse MAPK Phosphorylation Array

Référence AAH-MAPK-1-8

Marque : RayBiotech

Human/Mouse MAPK Phosphorylation Array