Human PLA2G2A (Phospholipase A2, Group IIA) Sandwich ELISA Kit Ready-To-Use

Storage Instruction:	The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	3 hrs

Gene Symbol:	PLA2G2A
Gene ID:	5320
Uniprot ID:	PA2GA_HUMAN
Immunogen Region:	Ready-To-Use
Sample Type:	serum, plasma, tissue homogenates or other biological fluids.

Tissue Specificity	Expressed in various tissues including heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, prostate, ovary, colon and small intestine. Not detected in lymphoid organs and brain. Expressed in platelets (at protein level).
Post Translational Modifications
Function	Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids with implications in host antimicrobial defense, inflammatory response and tissue regeneration. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. Contributes to lipid remodeling of cellular membranes and generation of lipid mediators involved in pathogen clearance. Displays bactericidal activity against Gram-positive bacteria by directly hydrolyzing phospholipids of the bacterial membrane. Upon sterile inflammation, targets membrane phospholipids of extracellular mitochondria released from activated platelets, generating free unsaturated fatty acids such as arachidonate that is used by neighboring leukocytes to synthesize inflammatory eicosanoids such as leukotrienes. Simultaneously, by compromising mitochondrial membrane integrity, promotes the release in circulation of potent damage-associated molecular pattern molecules that activate the innate immune response. Plays a stem cell regulator role in the intestinal crypt. Within intracellular compartment mediates Paneth cell differentiation and its stem cell supporting functions by inhibiting Wnt signaling pathway in intestinal stem cell (ICS). Secreted in the intestinal lumen upon inflammation, acts in an autocrine way and promotes prostaglandin E2 synthesis that stimulates Wnt signaling pathway in ICS cells and tissue regeneration. May play a role in the biosynthesis of N-acyl ethanolamines that regulate energy metabolism and inflammation. Hydrolyzes N-acyl phosphatidylethanolamines to N-acyl lysophosphatidylethanolamines, which are further cleaved by a lysophospholipase D to release N-acyl ethanolamines. Independent of its catalytic activity, acts as a ligand for integrins. Binds to and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1. Binds to a site (site 2) which is distinct from the classical ligand-binding site (site 1) and induces integrin conformational changes and enhanced ligand binding to site 1. Induces cell proliferation in an integrin-dependent manner.
Protein Name	Phospholipase A2 - Membrane Associated Giic Spla2 Group Iia Phospholipase A2 Non-Pancreatic Secretory Phospholipase A2 Nps-Pla2 Phosphatidylcholine 2-Acylhydrolase 2a
Database Links	Reactome: R-HSA-1482788 Reactome: R-HSA-1482801 Reactome: R-HSA-1482839 Reactome: R-HSA-1482922 Reactome: R-HSA-1482925 Reactome: R-HSA-1483166 Reactome: R-HSA-6803157
Cellular Localisation	Secreted Cell Membrane Peripheral Membrane Protein Mitochondrion Outer Membrane
Alternative ELISA Names	Phospholipase A2 - Membrane Associated ELISA kit Giic Spla2 ELISA kit Group Iia Phospholipase A2 ELISA kit Non-Pancreatic Secretory Phospholipase A2 ELISA kit Nps-Pla2 ELISA kit Phosphatidylcholine 2-Acylhydrolase 2a ELISA kit PLA2G2A ELISA kit PLA2B ELISA kit PLA2L ELISA kit RASF-A ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance