Human PLA2G3 (Phospholipase A2, Group III) Sandwich ELISA Kit Ready-To-Use

Storage Instruction:	The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	3 hrs

Gene Symbol:	PLA2G3
Gene ID:	50487
Uniprot ID:	PA2G3_HUMAN
Immunogen Region:	Ready-To-Use
Sample Type:	serum, plasma or other biological fluids.

Tissue Specificity	Expressed in kidney, heart, liver, and skeletal muscle. Also present in placenta and peripheral blood leukocytes. Not detected in colon, thymus, spleen and small intestine. In lung, expressed in bronchial epithelial cells and alveolar macrophages, but scarcely detected in alveolar epithelium, arterial walls and interstitial fibroblasts (at protein level). In joints of osteoarthritis and rheumatoid arthritis, expressed in endothelial cells (at protein level). In normal heart, detected in some vessels. In myocardial tissues with acute infarction, expressed in vascular endothelial cells adjacent to cardiomyocytes and those in lesions with granulation. Expression in cardiomyocytes is scarce (at protein level). In uterus, breast and colon cancers, detected in tumor cells and neighboring microvascular endothelium, but not in normal glandular tissues (at protein level). Expressed in dermal resting mast cells (at protein level) and pulmonary mast cells. Expressed in neuronal fibers (at protein level). Highly expressed in dorsal root ganglia neurons (at protein level). Expressed in Purkinje cells in cerebellum (at protein level). In stomach is preferentially expressed in neuronal fibers and in microvascular endothelium. Sparsely expressed in normal aorta (at protein level). Highly expressed in macrophages and smooth muscle cells in aorta with atheroma.
Post Translational Modifications	N-glycosylation does not affect the catalytic activity, but is required for proper secretion. A nonglycosylated form is observed in several cell types. In several cell types, the N- and C-termini are cleaved off.
Function	Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids without apparent head group selectivity. Contributes to phospholipid remodeling of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) particles. Hydrolyzes LDL phospholipids releasing unsaturated fatty acids that regulate macrophage differentiation toward foam cells. May act in an autocrine and paracrine manner. Secreted by immature mast cells, acts on nearby fibroblasts upstream to PTDGS to synthesize prostaglandin D2 (PGD2), which in turn promotes mast cell maturation and degranulation via PTGDR. Secreted by epididymal epithelium, acts on immature sperm cells within the duct, modulating the degree of unsaturation of the fatty acyl components of phosphatidylcholines required for acrosome assembly and sperm cell motility. Facilitates the replacement of fatty acyl chains in phosphatidylcholines in sperm membranes from omega-6 and omega-9 to omega-3 polyunsaturated fatty acids (PUFAs). Coupled to lipoxygenase pathway, may process omega-6 PUFAs to generate oxygenated lipid mediators in the male reproductive tract. At pericentrosomal preciliary compartment, negatively regulates ciliogenesis likely by regulating endocytotic recycling of ciliary membrane protein. Coupled to cyclooxygenase pathway provides arachidonate to generate prostaglandin E2 (PGE2), a potent immunomodulatory lipid in inflammation and tumorigenesis. At colonic epithelial barrier, preferentially hydrolyzes phospholipids having arachidonate and docosahexaenoate at sn-2 position, contributing to the generation of oxygenated metabolites involved in colonic stem cell homeostasis. Releases C16:0 and C18:0 lysophosphatidylcholine subclasses from neuron plasma membranes and promotes neurite outgrowth and neuron survival.
Protein Name	Group 3 Secretory Phospholipase A2 Group Iii Secretory Phospholipase A2 Giii Spla2 Spla2-Iii Phosphatidylcholine 2-Acylhydrolase 3
Database Links	Reactome: R-HSA-1482788 Reactome: R-HSA-1482839 Reactome: R-HSA-1482925
Cellular Localisation	Secreted Cell Membrane Cytoplasm Cytoskeleton Microtubule Organizing Center Centrosome Centriole Recycling Endosome Localized At Pericentrosomal Preciliary Compartment
Alternative ELISA Names	Group 3 Secretory Phospholipase A2 ELISA kit Group Iii Secretory Phospholipase A2 ELISA kit Giii Spla2 ELISA kit Spla2-Iii ELISA kit Phosphatidylcholine 2-Acylhydrolase 3 ELISA kit PLA2G3 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance