HUMAN Stem Cell Factor - (h-SCF)

Référence 100-04-10UG

Conditionnement : 10ug

Marque : Shenandoah

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Recombinant Human SCF

Catalog ID
100-04
Size
10 µg 100-04-10UG
10 µg (Animal-Free) 100-04AF-10UG
100 µg 100-04-100UG
100 µg (Animal-Free) 100-04AF-100UG
500 µg 100-04-500UG
500 µg (Animal-Free) 100-04AF-500UG
1 mg 100-04-1MG
1 mg (Animal-Free) 100-04AF-1MG
Details

Stem cell factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor c-Kit/CD117 and plays a critical role in the maintenance, survival, and differentiation of hematopoietic stem cells. While human SCF shows no activity on murine cells, murine and rat SCF are active on human cells.

Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.

Instructions

Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Technical Specifications

Alternate Names c-Kit Ligand, KL, Steel Factor, MGF
Accession Number P21583
Source Genetically modified E.coli
Predicted Molecular Mass Monomer, 18.6 kDa (165 aa)
AA Sequence MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA
Formulation Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
Product Reconstitution Sterile water at 0.1 mg/mL
Country of Origin USA

 

Specification* Method of Determination Acceptance Criteria
Purity Reducing and Non-Reducing SDS PAGE ≥ 97%
Endotoxin Kinetic LAL ≤ 0.1 EU/µg
Biological Activity (ED50) TF-1 cell proliferation ≤ 15 ng/mL; ≥ 6.7 x 104 units/mg

 

*Lot-specific values for the above specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements.

Specifications

Storage
At or below -20°C
Shelf Life
12 months from date of receipt when stored at -20°C to-80°C as supplied.

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