Human TRPA1 (Transient Receptor Potential Cation Channel Subfamily A, Member 1) Sandwich ELISA Kit E0012813)

Storage Instruction:	Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	4.5 hrs

Gene Symbol:	TRPA1
Gene ID:	8989
Uniprot ID:	TRPA1_HUMAN
Sample Type:	tissue homogenates, cell lysates or other biological fluids.

Tissue Specificity	Expressed at very low levels in fibroblasts.
Post Translational Modifications	TRPA1 activation by electrophiles occurs though covalent modification of specific cysteine residues in the N-terminal cytoplasmic domain. Hydroxylation is required for TRPA1 activity inhibition in normoxia. In hypoxia, the decrease in oxygen concentration diminishes the activity of the hydroxylase EGLN1, thus relieving TRPA1 from inhibition and ultimately leading to channel activation. Oxidation of Cys-633 and Cys-856 in hyperoxia may override the hydroxylase EGLN1-mediated inhibition, causing TRPA1 activation.
Function	Receptor-activated non-selective cation channel involved in pain detection and possibly also in cold perception, oxygen concentration perception, cough, itch, and inner ear function. Shows 8-fold preference for divalent over monovalent cations. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of irritants, such as allylthiocyanate (AITC) from mustard oil or wasabi, cinnamaldehyde, diallyl disulfide (DADS) from garlic, and acrolein, an irritant from tears gas and vehicle exhaust fumes. Acts also as an ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Is activated by a large variety of structurally unrelated electrophilic and non-electrophilic chemical compounds. Electrophilic ligands activate TRPA1 by interacting with critical N-terminal Cys residues in a covalent manner, whereas mechanisms of non-electrophilic ligands are not well determined. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system.
Protein Name	Transient Receptor Potential Cation Channel Subfamily A Member 1 Ankyrin-Like With Transmembrane Domains Protein 1 Transformation-Sensitive Protein P120 P120 Wasabi Receptor
Database Links	Reactome: R-HSA-3295583
Cellular Localisation	Cell Membrane Multi-Pass Membrane Protein
Alternative ELISA Names	Transient Receptor Potential Cation Channel Subfamily A Member 1 ELISA kit Ankyrin-Like With Transmembrane Domains Protein 1 ELISA kit Transformation-Sensitive Protein P120 ELISA kit P120 ELISA kit Wasabi Receptor ELISA kit TRPA1 ELISA kit ANKTM1 ELISA kit
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Information sourced from Uniprot.org

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