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> IgG, Kappa (Biotin)

IgG, Kappa (Biotin)

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Référence I1903-36D-1mg

Conditionnement : 1mg

Marque : US Biological


I1903-36D IgG, Kappa (Biotin)

Clone Type
Polyclonal
Host
goat
Source
human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:2000-1:10,000
ELISA: 1:200,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Label:
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)

Biotin/Protein Ratio:
10-20 BAC molecules per goat IgG molecule

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Product Type: Pab|Isotype: IgG|Host: goat|Source: human|Concentration: ~1mg/ml (after reconstitution)|Form: Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Reconstitute with 1ml sterile dH2O. Labeled with Biotin.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Human kappa light chain|Specificity: Recognizes the kappa chain subunit of IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-goat serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other human heavy or light chain proteins.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Human kappa light chain
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Reconstitute with 1ml sterile dH2O. Labeled with Biotin.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes the kappa chain subunit of IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-goat serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other human heavy or light chain proteins.
References
1. Bayer & Wilchek, Methods in Enzymology 184: 138-160 (1990).