IgG (TRITC)

Référence I1903-35E-1mg

Conditionnement : 1mg

Marque : US Biological



I1903-35E IgG (TRITC)

Clone Type
Polyclonal
Host
goat
Source
human
Isotype
IgG
Grade
Affinity Purified
Applications
E IF WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C (-20°C Glycerol)

Applications:
Suitable for use in ELISA and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Absorption Wavelength:
550nm

Emission Wavelength:
570nm

Fluorochrome/Protein Ratio:
3.0 moles TRITC per mole of Goat IgG

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Product Type: Pab|Isotype: IgG|Host: goat|Source: human|Concentration: ~1mg/ml|Form: Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Label: Tetramethylrhodamine isothiocyanate (TRITC) (MW 444d). Reconstitute to 1ml in sterile dH2O or 40-50% glycerol.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Human IgG heavy chain|Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, human IgG, and human serum. Less than 1% crossreactivity was observed by ELISA against other human heavy and light chain proteins. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Human IgG heavy chain
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Label: Tetramethylrhodamine isothiocyanate (TRITC) (MW 444d). Reconstitute to 1ml in sterile dH2O or 40-50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, human IgG, and human serum. Less than 1% crossreactivity was observed by ELISA against other human heavy and light chain proteins.
References
1. Titus, J., et al., J. Immunol. Methods 50: 193 (1982).