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Sandwich ELISA
Application

Sandwich ELISA

Sandwich ELISA using IgG1 monoclonal antibody, clone KT46 (Cat # MAB9724) coated plate and HRP conjugated IgG monoclonal antibody, clone KT47 (Cat # MAB9725).

QC Test

  • Specification

    Product Description

    Rat monoclonal antibody raised against recombinant human IgG1 Fc.

    Immunogen

    Recombinant fusion protein corresponding to human IgG1 Fc.

    Host

    Rat

    Reactivity

    Human

    Specificity

    This antibody is specific to human IgG1. Not cross-react with human IgG2, IgG3 and IgG4, IgA, IgE and IgM.

    Form

    Liquid

    Purification

    Protein G purification

    Concentration

    1 mg/mL

    Isotype

    IgG2a

    Quality Control Testing

    Cross-reactivity test

    Recommend Usage

    ELISA (1 ug/mL)
    Sandwich ELISA (5 ug/mL for coating)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (0.09% sodium azide)

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C or -80°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Enzyme-linked Immunoabsorbent Assay

    Sandwich ELISA

    Sandwich ELISA using IgG1 monoclonal antibody, clone KT46 (Cat # MAB9724) coated plate and HRP conjugated IgG monoclonal antibody, clone KT47 (Cat # MAB9725).