Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.

IgG2 (MaxLight 490)

Imprimer

Référence I1904-80F-ML490-100ul

Conditionnement : 100ul

Marque : US Biological

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I1904-80F-ML490 IgG2 (MaxLight 490)

Clone Type

Monoclonal

Host

mouse

Source

human

Swiss Prot

P01859

Isotype

IgG1,k

Grade

Purified

Applications

FLISA IHC

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

Applications:
Suitable for use in Immunohistochemistry and FLISA. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Recommended Negative Control:
I1904-78R: IgG1 Murine Negative Control

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG1,k|Clone No: 3H2343 (HP6014)|Host: mouse|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.|Purity: Purified|Immunogen: Purified human IgG2|Specificity: Recognizes human IgG2 at an epitope localized to the Fab region. Will not cross-react with IgG1, IgG3, IgG4, free kappa, free lambda, IgM, IgA1, IgA2 or IgD as tested by hemagglutination assays.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Purified human IgG2

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.

Purity

Purified

Specificity

Recognizes human IgG2 at an epitope localized to the Fab region. Will not cross-react with IgG1, IgG3, IgG4, free kappa, free lambda, IgM, IgA1, IgA2 or IgD as tested by hemagglutination assays.

References

1. Jefferis, R. et al. (1985). Evaluation of Monoclonal Antibodies having specificity for Human IgG sub-classes. Results of an IUIS/WHO collaborative study. Immunology Letters. 10: 223-252.2. Hussain, R. et al. (1986). 2. Hussain, R. et al . (1986). Use of monoclonal antibodies to quantify subclasses of human IgG. J. Immunol. Meth. 93: 89-96. 3. Jefferis, R. et al . (1988). Immunology. 63: 171.