IHC-plus™ GLI3 Antibody
Référence LS-B73-50
Conditionnement : 50ug
Marque : LSBio
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Téléphone : +1 850 650 7790
Overview
Antibody:
GLI3 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Chimpanzee, Dog, Chicken, Quail, Squirrel monkey, Xenopus laevis
Format:
Unconjugated, Unmodified
Specifications
Description
GLI3 antibody LS-B73 is an unconjugated rabbit polyclonal antibody to GLI3 from human. It is reactive with human, chicken, chimpanzee and other species. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human GLI3
Synonyms
GLI3 | ACLS | GCPS | GLI-Kruppel family member GLI3 | GLI3 full length protein | GLI3-190 | GLI family zinc finger 3 | Gli-3 | GLI3FL | PAP-A | PAPA1 | PHS | PPDIV | Transcriptional activator GLI3 | PAPA | PAPB | GLI3 form of 190 kDa | Oncogene GLI3 | Zinc finger protein GLI3
Host
Rabbit
Reactivity
Human, Chimpanzee, Dog, Chicken, Quail, Squirrel monkey, Xenopus laevis (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Dog, Chicken, Quail, Squirrel monkey, Xenopus laevis (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was produced from monospecific rabbit serum by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-60 of human Gli-3 protein.
Specificity
A BLAST analysis was used to suggest cross reactivity with Gli-3 from human, chimpanzee, squirrel monkey, Xenopus laevis, chicken, dog and quail based on 100% sequence homology with the immunogen. Reactivity is also expected against homologues from mouse (94%) and rat (88%) based on partial homology. Reactivity with Gli-3 from other sources is not known.
Applications
- IHC
- IHC - Paraffin (1.25 µg/ml)
- Immunofluorescence
- Western blot (1:500 - 1:2000)
- ELISA (1:6000 - 1:30000)
Usage
Immunohistochemistry: LS-B73 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B73 was determined to be 1.25 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee About GLI3
P10071 NM_000168 NP_000159.3
Validation
Anti-GLI3 antibody IHC of human brain-glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1.25 ug/ml.
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Cond.
Prix HT
