IL-33 ELISA Kit (Mouse)
Référence OKAG00188
Conditionnement : 1plate
Marque : Aviva Systems Biology
IL-33 ELISA Kit (Mouse) : 96 Wells (OKAG00188)
| Datasheets/Manuals | Printable datasheet for IL-33 ELISA Kit (Mouse) : 96 Wells (OKAG00188) |
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| Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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| Product Format | 1 x 96-Well Plate or 12 x 8-Well Strips | ||||||||||||||||||||||
| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | 4.5 hours | ||||||||||||||||||||||
| ELISA Kit Principle | The Aviva Murine IL-33 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant mIL-33 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Murine IL-33 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration. | ||||||||||||||||||||||
| ELISA Kit Range | 16-1000 pg/mL | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C | ||||||||||||||||||||||
| Sample Type | Cell lysates, sera and plasma | ||||||||||||||||||||||
| Sensitivity | 16 pg/mL | ||||||||||||||||||||||
| Specificity | The Murine IL-33 ELISA is capable of recognizing both recombinant and naturally produced Murine IL-33 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Murine: Pro-IL-33 (108 aa), ST2/IL-1 R4/Fc Chimera | ||||||||||||||||||||||
| Assay Info | Quantitative Colorimentric Sandwich ELISA |
| Gene Symbol | Il33 |
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| Gene Full Name | interleukin 33 |
| Alias Symbols | 9230117N10Rik, Il-, Il-33, Il1f, Il1f11, interleukin-33, NF-H, NF-HEV, nuclear factor from high endothelial venules. |
| NCBI Gene Id | 77125 |
| Protein Name | Interleukin-33 |
| Description of Target | Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury. |
| Uniprot ID | Q8BVZ5 |
| Protein Accession # | NP_001158196.1 |
| Nucleotide Accession # | NM_001164724.1 |
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