OKEH00140 | IL6 ELISA Kit (Human) Clinisciences

Référence OKEH00140

Conditionnement : 96Wells

Contactez votre distributeur local :

Téléphone : +1 850 650 7790

Marque : Aviva Systems Biology

Contactez votre distributeur local :

Téléphone : +1 850 650 7790

Datasheets/Manuals	Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.

Product Info

Predicted Species Reactivity

Homo sapiens, Human

Application

Enzyme-linked Immunosorbent assay-Sandwich

ELISA Kit Detection Method

Colorimetric, OD 450 nm

ELISA Kit Duration

~ 3 Hours

ELISA Kit Principle

Aviva Systems Biology IL6 ELISA Kit (Human) (OKEH00140) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL6 captured in well.

ELISA Kit Range

15.6-1000pg/mL

ELISA Kit Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%

ELISA Kit Reproducibility

Mean Intra-assay CV%: < 5.6% (n = 20)
Mean Inter-assay CV%: < 9.2% (n = 20)

ELISA Kit Component

Component	Amount
IL6 Microplate	96 Wells (12 x 8 Well strips)
IL6 Lyophilized Standard	2
100X Biotinylated IL6 Detector Antibody	1 x 120 uL
100X Avidin-HRP Conjugate	1 x 120 uL
Sample Diluent	1 x 20 mL
Detector Antibody Diluent	1 x 12 mL
Conjugate Diluent	1 x 12 mL
25X Wash Buffer	1 x 30 mL
TMB Substrate	1 x 10 mL
Stop Solution	1 x 10 mL

Reconstitution and Storage

Store as indicated in product manual.

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Sensitivity

9.8 pg/mL

Specificity

Natural and recombinant Human Interleukin-6

Assay Info

Assay Methodology: Quantitative Sandwich ELISA

Gene Symbol	IL6
Gene Full Name	interleukin 6
Alias Symbols	B-cell differentiation factor;B-cell stimulatory factor 2;BSF2;BSF-2;CDF;CTL differentiation factor;HGF;HSF;hybridoma growth factor;IFNB2;IFN-beta-2;IL-6;interferon beta-2;interleukin BSF-2;interleukin-6.
NCBI Gene Id	3569
Protein Name	P05231
Description of Target	Interleukin-6
Uniprot ID	https://www.uniprot.org/uniprot/P05231
Protein Accession #	NP_000591
Nucleotide Accession #	NM_000600
Protein Size (# AA)	212
Molecular Weight	24 kDa

Biological pathways

Name	# of Products
Acute-phase response	209
Cellular response to hydrogen peroxide	43
Defense response to Gram-negative bacterium	93
Defense response to Gram-positive bacterium	56
Defense response to virus	284
Endocrine pancreas development	246
Glucagon secretion	22
Hepatic immune response	18
Humoral immune response	159
Interleukin-6-mediated signaling pathway	33
Monocyte chemotaxis	65
Negative regulation of apoptotic process	538
Negative regulation of cell proliferation	554
Negative regulation of chemokine biosynthetic process	28
Negative regulation of collagen biosynthetic process	20
Negative regulation of fat cell differentiation	103
Negative regulation of lipid storage	119
Neuron projection development	76
Neutrophil apoptosis	32
Neutrophil mediated immunity	28
Platelet activation	875
Positive regulation of acute inflammatory response	31
Positive regulation of anti-apoptosis	118
Positive regulation of B cell activation	24
Positive regulation of chemokine production	128
Positive regulation of immunoglobulin secretion	60
Positive regulation of interleukin-6 production	149
Positive regulation of leukocyte chemotaxis	30
Positive regulation of osteoblast differentiation	109
Positive regulation of peptidyl-serine phosphorylation	168
Positive regulation of sequence-specific DNA binding transcription factor activity	174
Positive regulation of smooth muscle cell proliferation	123
Positive regulation of STAT protein import into nucleus	20
Positive regulation of T cell proliferation	211
Positive regulation of transcription from RNA polymerase II promoter	1210
Positive regulation of translation	71
Positive regulation of tyrosine phosphorylation of Stat3 protein	101
Regulation of angiogenesis	60
Regulation of vascular endothelial growth factor production	22
Response to glucocorticoid stimulus	111
Response to peptidoglycan	18

IL6 ELISA Kit (Human) : 96 Wells (OKAA00012-96W)
Catalog #: OKAA00012-96W

Application: ELISA

Sensitivity: 2 pg/ml

Size: 96 Wells
Interleukin-6 (Western Blot Control) Protein (OPRB00205)
Catalog #: OPRB00205

Application: Control|Western blot

Format: Liquid. Supplied in 1x Laemmli Buffer (25mM Tris-HCl pH6.8, 50mM DTT, 1% (w/v) SDS, 0.1% (w/v) Bromophenol Blue, 2.5% Glycerol).

Size: 10UG
IL-6 ELISA Kit (Human) : 96 Wells (OKAG00041)
Catalog #: OKAG00041

Application: Enzyme-linked Immunosorbent assay-Sandwich

Kit Range: 32-2000 pg/ml

Sensitivity: 32 pg/mL

Format: 1 x 96-Well Plate or 12 x 8-Well Strips

Size: 12x8WELLS
IL6 ELISA Kit (Rat) : 96 Wells (OKEH00143)
Catalog #: OKEH00143

Application: Enzyme-linked Immunosorbent assay-Sandwich

Kit Range: 15.6-1000pg/mL

Sensitivity: 7.81 pg/mL

Size: 96T
IL6 ELISA Kit (Pig) (OKEH03991)
Catalog #: OKEH03991

Application: Enzyme-linked Immunosorbent assay-Sandwich

Kit Range: 78-5000pg/mL

Sensitivity: 18 pg/mL

Size: 96T

IL6 ELISA Kit (Human)

Référence OKEH00140

Contactez votre distributeur local :

Marque : Aviva Systems Biology

Contactez votre distributeur local :

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