Immunoglobulin G2

There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). Human IgG2 subclass has been identified to exist as an ensemble of distinct isoforms, designated IgG2-A, IgG2-B, and IgG2-A/B, which differ by the disulfide connectivity at the hinge region. IgG2, the second largest part of IgG isotypes in humans, comprises 20-25% of the main subclass and is the prevalent immune response against carbohydrate-/polysaccharid antigens.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.