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> Influenza A virus PA protein antibody

Influenza A virus PA protein antibody

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Identifiez-vous

Référence GTX118991-100ul

Conditionnement : 100ul

Marque : Genetex

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Téléphone : +1 850 650 7790

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Anti-Influenza A virus PA protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118991
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
10

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Influenza A virus
Package
100 μl,
25 μl
Datasheet File
View product citations for antibody GTX118991 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:1000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

DF-1(WSN infected cell s)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.91 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Influenza A virus PA (A/WSN/1933(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619959

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza A Virus Polymerase acidic protein , Influenza A Virus PA

DATA IMAGES

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991

GTX118991 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (- μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PA protein antibody (GTX118991) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PA protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118991

GTX118991 ICC/IF Image

Influenza A Virus PA antibody detects polymerase PA (influenza A H1N1) protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: polymerase PA (influenza A H1N1) protein stained by Influenza A Virus PA antibody (GTX118991) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991

GTX118991 WB Image

Sample (60 μg of whole cell lysate)
A: DF-1(WSN infected cell lysates)
B: DF-1 cell lysates
GTX118991 diluted at 1:250
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Application Reference

WK Cho et al. J Funct ?Foods 2024; 112 : 105940 Hoveniae Semen Seu Fructus water extract inhibits influenza A virus infection
Application : ICC/IF
Sheppard CM et al. Nat Commun 2023; 14 (1) : 6135 An Influenza A virus can evolve to use human ANP32E through altering polymerase dimerization.
Han YJ et al. J Biomed Sci 2023; 30 (1) : 10 Targeting influenza A virus by splicing inhibitor herboxidiene reveals the importance of subtype-specific signatures around splice sites.
Li Q et al. Adv Sci (Weinh) 2022; 9 (34) : e2203088 SRSF5-Mediated Alternative Splicing of M Gene is Essential for Influenza A Virus Replication: A Host-Directed Target Against Influenza Virus.
Sonja Fernbach et al. Cell Rep 2022; 38 (12) : 110549 Restriction factor screening identifies RABGAP1L-mediated disruption of endocytosis as a host antiviral defense.
Application : WB
Submit a Reference

REVIEW

Influenza A virus PA protein antibody Cat. No. GTX118991

Rating
( Average 5 based on 10 users reviews)
Application
Western Blot(WB)
( Average 5 based on 9 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Date : Chen-I Hsu submitted on 10-Sep-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Secondary Antibody : GTX213110-01, 1:2000, 25°C, 1Hr
Lane Description :
Left : A549 cells were transfected with pcDNA3-PA (WSN) using Lipofectamine3000. The cell was harvested at 24h post-transfection.
Right : A549 cells were infected with Influenza A (WSN) (MOI=0.1) and harvested at 6, 24, 48 hours postinfection.
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 29118119
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 30209172
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 29118119
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 30209172
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28842649
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2015. PMID: 25652161
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2015. PMID: 25652161
Anti-Influenza A virus PA protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118991
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2015. PMID: 25652161
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX118991
Date : Anonymous submitted on 15-Jun-2015
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : influenza virus (A/WSN/33) infected MDCK cells
Amount used : 3 x 10^3 cells
Blocking : 5% nonfat drymilk in TBST, 25°C, overnightHr
Primary Antibody : 1:5000, 25°C, 4Hr
Lane Description : Lane 1: influenza virus (A/WSN/33) infected MDCK cells (MOI=1 9hr). Lane 2: MDCK cells.
Submit a Review
Application Reference
WK Cho et al. J Funct ?Foods 2024; 112:105940 Hoveniae Semen Seu Fructus water extract inhibits influenza A virus infection
Application : ICC/IF
Sheppard CM et al. Nat Commun 2023; 14 (1):6135 An Influenza A virus can evolve to use human ANP32E through altering polymerase dimerization.
Han YJ et al. J Biomed Sci 2023; 30 (1):10 Targeting influenza A virus by splicing inhibitor herboxidiene reveals the importance of subtype-specific signatures around splice sites.
Li Q et al. Adv Sci (Weinh) 2022; 9 (34):e2203088 SRSF5-Mediated Alternative Splicing of M Gene is Essential for Influenza A Virus Replication: A Host-Directed Target Against Influenza Virus.
Sonja Fernbach et al. Cell Rep 2022; 38 (12):110549 Restriction factor screening identifies RABGAP1L-mediated disruption of endocytosis as a host antiviral defense.
Application : WB
Ma C et al. PLoS Pathog 2022; 18 (2):e1010295 p21 restricts influenza A virus by perturbing the viral polymerase complex and upregulating type I interferon signaling.
Application : WB
Hongmin Sun et al. Engineering (Beijing) 2021; Multi-Omics-Guided Discovery of Omicsynins Produced by Streptomyces sp. 1647: Pseudo-Tetrapeptides Active Against Influenza A Viruses and Coronavirus HCoV-229E.
Application : WB
Bdeir N et al. Sci Rep 2021; 11 (1):20477 Evidence that two instead of one defective interfering RNA in influenza A virus-derived defective interfering particles (DIPs) does not enhance antiviral activity.
Application : WB
Liu X et al. Nat Commun 2021; 12 (1):4427 MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.
Application : WB
Gong W et al. J Virol 2021; Interaction of NEP with G Protein Pathway Suppressor 2 Facilitates Influenza A Virus Replication by Weakening the Inhibition of GPS2 to RNA Synthesis and Ribonucleoprotein Assembly.
Application : WB
Kwon EB et al. Molecules 2021; 26 (4) 8-O-(E-p-methoxycinnamoyl)harpagide Inhibits Influenza A Virus Infection by Suppressing Intracellular Calcium.
Application : WB
Zhu Y et al. Sci Adv 2020; 6 (25):eaaz5764 Human TRA2A determines influenza A virus host adaptation by regulating viral mRNA splicing.
Sun N et al. Cell Rep 2020; 33 (5):108342 Type-IInterferon-Inducible SERTAD3 Inhibits Influenza A Virus Replication by Blocking the Assembly of Viral RNA Polymerase Complex.
Application : WB
Esparza M et al. PLoS Pathog 2020; 16 (4):e1008407 Chemical intervention of influenza virus mRNA nuclear export.
Gao Q et al. J Virol 2020; 95 (2) Eukaryotic Translation Elongation Factor 1 Delta Inhibits the Nuclear Import of the Nucleoprotein and PA-PB1 Heterodimer of Influenza A Virus.
Application : WB
Arai Y et al. J Virol 2020; 95 (1) PA Mutations Inherited during Viral Evolution Act Cooperatively To Increase Replication of Contemporary H5N1 Influenza Virus with an Expanded Host Range.
Application : WB
Hu J et al. Vet Microbiol 2020; 250:108838 PA-X protein of H5N1 avian influenza virus inhibits NF-kappaB activity, a potential mechanism for PA-X counteracting the host innate immune responses.
Application : WB, ICC/IF
Zhang X et al. Front Microbiol 2020; 11:538355 Avian Chaperonin Containing TCP1 Subunit 5 Supports Influenza A Virus Replication by Interacting With Viral Nucleoprotein, PB1, and PB2 Proteins.
Application : WB
Hu A et al. Front Microbiol 2020; 11:178 Anthralin Suppresses the Proliferation of Influenza Virus by Inhibiting the Cap-Binding and Endonuclease Activity of Viral RNA Polymerase.
Application : WB
Domingues P et al. Nat Commun 2019; 10 (1):3396 Profiling host ANP32A splicing landscapes to predict influenza A virus polymerase adaptation.
Application : WB
Wang R et al. J Virol 2019; 93 (4) Autophagy Promotes Replication of Influenza A Virus In Vitro.
Chao TL et al. Front Microbiol 2019; 10:2942 Characterization of Influenza A Virus Infection in Mouse Pulmonary Stem/Progenitor Cells.
Application : WB
Rodriguez-Frandsen A et al. J Virol 2019; (Epub) Viral determinants in H5N1 influenza A virus enable productive infection of HeLa cells.
Application : WB
Hao X et al. Transbound Emerg Dis 2019; (Epub) The PB2 and M genes are critical for the superiority of genotype S H9N2 virus to genotype H in optimizing viral fitness of H5Nx and H7N9 avian influenza viruses in mice.
Application : WB
Choi JG et al. Sci Rep 2019; 9 (1):12132 Antiviral activity of ethanol extract of Geranii Herba and its components against influenza viruses via neuraminidase inhibition.
Application : WB
Arai Y et al. PLoS Pathog 2019; 15 (7):e1007919 PB2 mutations arising during H9N2 influenza evolution in the Middle East confer enhanced replication and growth in mammals.
Application : WB
Hao X et al. Virology 2019; 535:218-226 The PB2 and M genes of genotype S H9N2 virus contribute to the enhanced fitness of H5Nx and H7N9 avian influenza viruses in chickens.
Application : WB
Zhao L et al. Mol Ther Nucleic Acids 2019; 14:509-519 The Downregulation of MicroRNA hsa-miR-340-5p in IAV-Infected A549 Cells Suppresses Viral Replication by Targeting RIG-I and OAS2.
Application : WB
Lin HT et al. Sci Rep 2018; 8 (1):17242 Grail attenuates influenza A virus infection and pathogenesis by inhibiting viral nucleoprotein.
Application : WB
Yang C et al. J Virol 2018; (Epub) The nucleolar protein LYAR facilitates ribonucleoprotein assembly of influenza A virus.
Application : WB, ICC/IF
Mizuta S et al. ChemMedChem 2018; (Epub) Synthesis of Trifluoromethyl-α,β-unsaturated Lactones and Pyrazolinones and Discovery of Influenza Virus Polymerase Inhibitors.
Application : WB
Wang J et al. Viruses 2018; 10 (6) Host Long Noncoding RNA lncRNA-PAAN Regulates the Replication of Influenza A Virus.
Application : WB
Chen SC et al. J Virol 2017; 91 (20) Zinc Finger-Containing Cellular Transcription Corepressor ZBTB25 Promotes Influenza Virus RNA Transcription and Is a Target for Zinc Ejector Drugs.
Oymans J et al. J Virol 2017; (Epub) A mechanism for prime-realignment during influenza A virus replication.
Application : WB
Oymans J et al. bioRxiv 2017; (Epub) A mechanism for prime-realignment during influenza A virus replication.
Application : WB
Kuss-Duerkop SK et al. PLoS Pathog 2017; 13 (9):e1006635 Influenza virus differentially activates mTORC1 and mTORC2 signaling to maximize late stage replication.
Watanabe K et al. Sci Rep 2017; 7 (1):9500 Structure-based drug discovery for combating influenza virus by targeting the PA-PB1 interaction.
Application : WB
Xu G et al. J Virol 2016; (Epub) Prevailing PA mutation K356R in avian influenza H9N2 virus increases mammalian replication and pathogenicity.
Gao H et al. Sci Rep 2015; 5:8262 The contribution of PA-X to the virulence of pandemic 2009 H1N1 and highly pathogenic H5N1 avian influenza viruses.
Application : WB, ICC/IF
Su WC et al. J Virol 2015; 89 (22):11245-55 A Nucleolar Protein, Ribosomal RNA Processing 1 Homolog B (RRP1B), Enhances the Recruitment of Cellular mRNA in Influenza Virus Transcription.
Application : WB
Hsu SF et al. J Virol 2015; 89 (7):3671-82 A Host Susceptibility Gene, DR1, Facilitates Influenza A Virus Replication by Suppressing Host Innate Immunity and Enhancing Viral RNA Replication.
Application : WB
Roberts KL et al. J Virol 2014; Influenza A virus uses intercellular connections to spread to neighboring cells.
Application : ICC/IF
Hsu WB et al. J Virol 2013; 87 (1):110-23 Cellular protein HAX1 interacts with the influenza A virus PA polymerase subunit and impedes its nuclear translocation.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf