Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> IPAF / NLRC4 Antibody

IPAF / NLRC4 Antibody

Imprimer Imprimer
Identifiez-vous

Référence orb1536902-50ul

Conditionnement : 50ul

Marque : Biorbyt

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

    • Home
    • IPAF / NLRC4 Antibody
    IPAF / NLRC4 Antibody

    IPAF / NLRC4 Antibody

    Catalog Number: orb1536902

    Catalog Numberorb1536902
    CategoryAntibodies
    DescriptionIPAF / NLRC4 Antibody
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsICC, IF, IHC-P, WB
    ReactivityHuman, Mouse, Rat
    ImmunogenRecombinant full length protein of human CARD12
    Concentration1 mg/ml
    Dilution rangeICC (1:50 - 1:200), IF (1:50 - 1:200), IHC-P (1:50 - 1:200), WB (1:500 - 1:2000)
    ConjugationUnconjugated
    TargetIPAF / NLRC4
    StorageAliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.. Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesNLRC4, CARD12, CLANA, CLANB, CLANC, CLAND, CLAN, C
    NoteFor research use only
    Application notesFurther information: Western blot analysis of CARD12 expression in MCF7 (A), mouse heart (B), rat kidney (C) whole cell lysates. Immunohistochemical analysis of CARD12 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Immunofluorescent analysis of CARD12 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    Expiration Date12 months from date of receipt.